Neuropeptide galanin increase ROS and IL-1β production by blood cells from patients with multiple sclerosis

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dc.creatorPedro Henrique Villar-Delfino
dc.creatorCaroline Maria Oliveira Volpe
dc.creatorThais Cristina Miranda
dc.creatorPaulo Pereira Christo
dc.creatorJose Augusto Nogueira-Machado
dc.date.accessioned2024-11-22T21:02:43Z
dc.date.accessioned2025-09-09T00:07:01Z
dc.date.available2024-11-22T21:02:43Z
dc.date.issued2018
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doi10.4172/2155-9899.1000555
dc.identifier.issn2155-9899
dc.identifier.urihttps://hdl.handle.net/1843/78223
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Clinical & Cellular Immunology
dc.rightsAcesso Aberto
dc.subjectEsclerose Múltipla
dc.subjectGalanina
dc.subjectEspécies Reativas de Oxigênio
dc.subjectInflamassomos
dc.subjectInflamação
dc.subjectImunidade Inata
dc.subjectGranulócitos
dc.titleNeuropeptide galanin increase ROS and IL-1β production by blood cells from patients with multiple sclerosis
dc.typeArtigo de periódico
local.citation.epage4
local.citation.issue4
local.citation.spage1
local.citation.volume9
local.description.resumoObjective: Galanin (GAL), a neuropeptide produced in the central and peripheral nervous system, has been involved in the modulation of the inflammatory response. Our present study aimed to evaluate the effect of GAL on the production of reactive oxygen species (ROS) by granulocytes and the secretion of IL-1β by peripheral blood mononuclear cells (PBMNC) from patients with Multiple Sclerosis (MS) and healthy control cells, comparatively. Methods: Granulocytes and PBMNC from patients with MS and healthy controls were purified simultaneously by the Ficoll-Hypaque gradient method. The effect of GAL on ROS generation by granulocytes was performed in a luminol-dependent chemiluminescence assay. The quantification of IL-1β in the supernatant of cultured-PBMNC was determined using immunoassay (ELISA). Results: Our results showed that ROS production in the presence of GAL was significantly higher (p<0.05) in granulocytes of MS patients than that observed in healthy control cells. GAL activated IL-1β secretion similarly in both PBMNC of MS patients and healthy controls (p>0.05). Conclusion: GAL modulates the production of ROS and activates inflammasome evaluated by the increase of IL-1β secretion and it may have consequences in the inflammatory process observed in MS.
local.publisher.countryBrasil
local.publisher.departmentMED - DEPARTAMENTO DE CLÍNICA MÉDICA
local.publisher.initialsUFMG
local.url.externahttps://www.longdom.org/open-access/neuropeptide-galanin-increase-ros-and-il1-production-by-blood-cells-from-patients-with-multiple-sclerosis-52321.html

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