Single-colony sequencing reveals microbe-by-microbiome phylosymbiosis between the cyanobacterium Microcystis and its associated bacteria

Página do item simplificado
dc.creatorOlga Maria Pérez Carrascal
dc.creatorNicolas Tromas
dc.creatorYves Terrat
dc.creatorElisa Moreno
dc.creatorAlessandra Giani
dc.creatorLaisa Corrêa Braga Marques
dc.creatorNathalie Fortin
dc.creatorJesse Shapiro
dc.date.accessioned2023-06-23T22:58:18Z
dc.date.accessioned2025-09-08T23:54:29Z
dc.date.available2023-06-23T22:58:18Z
dc.date.issued2021
dc.identifier.doihttps://doi.org/10.1186/s40168-021-01140-8
dc.identifier.issn2049-2618
dc.identifier.urihttps://hdl.handle.net/1843/55308
dc.languagepor
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofMicrobiome
dc.rightsAcesso Aberto
dc.subjectCianobacteria
dc.subject.otherMicrocystis
dc.subject.otherCyanobacteria
dc.subject.otherPhylosymbiosis
dc.subject.otherCo-phylogeny
dc.subject.otherMicrobiome
dc.subject.otherPhycosphere
dc.titleSingle-colony sequencing reveals microbe-by-microbiome phylosymbiosis between the cyanobacterium Microcystis and its associated bacteria
dc.typeArtigo de periódico
local.citation.epage21
local.citation.issue1
local.citation.spage1
local.citation.volume9
local.description.resumoBackground: Cyanobacteria from the genus Microcystis can form large mucilaginous colonies with attached heterotrophic bacteria—their microbiome. However, the nature of the relationship between Microcystis and its microbiome remains unclear. Is it a long-term, evolutionarily stable association? Which partners benefit? Here we report the genomic diversity of 109 individual Microcystis colonies—including cyanobacteria and associated bacterial genomes—isolated in situ and without culture from Lake Champlain, Canada and Pampulha Reservoir, Brazil. Results: We identified 14 distinct Microcystis genotypes from Canada, of which only two have been previously reported, and four genotypes specific to Brazil. Microcystis genetic diversity was much greater between than within colonies, consistent with colony growth by clonal expansion rather than aggregation of Microcystis cells. We also identified 72 bacterial species in the microbiome. Each Microcystis genotype had a distinct microbiome composition, and more closely related genotypes had more similar microbiomes. This pattern of phylosymbiosis could be explained by co-phylogeny in only two out of the nine most prevalent associated bacterial genera, Roseomonas and Rhodobacter. These phylogenetically associated genera could enrich the metabolic repertoire of Microcystis, for example by encoding the biosynthesis of complementary carotenoid molecules. In contrast, other colony-associated bacteria showed weaker signals of co-phylogeny, but stronger evidence of horizontal gene transfer with Microcystis. These observations suggest that acquired genes are more likely to be retained in both partners (Microcystis and members of its microbiome) when they are loosely associated, whereas one gene copy is sufficient when the association is physically tight and evolutionarily long-lasting.Conclusions: We have introduced a method for culture-free isolation of single colonies from nature followed by metagenomic sequencing, which could be applied to other types of microbes. Together, our results expand the known genetic diversity of both Microcystis and its microbiome in natural settings, and support their long-term, specific, and potentially beneficial associations.
local.identifier.orcidhttps://orcid.org/0000-0003-3442-6192
local.identifier.orcidhttps://orcid.org/0000-0003-4332-8463
local.identifier.orcidhttps://orcid.org/0000-0002-2524-8534
local.identifier.orcidhttps://orcid.org/0000-0002-3653-0631
local.identifier.orcidhttps://orcid.org/0000-0003-2966-0611
local.identifier.orcidhttps://orcid.org/0000-0001-6819-8699
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE BOTÂNICA
local.publisher.initialsUFMG
local.url.externahttps://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-021-01140-8

Arquivos

Pacote original

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
Single-colony sequencing reveals microbe-by-microbiome phylosymbiosis between the cyanobacterium Microcystis and its associated bacteria.pdf
Tamanho:
11.37 MB
Formato:
Adobe Portable Document Format
Baixar

Licença do pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
License.txt
Tamanho:
1.99 KB
Formato:
Plain Text
Descrição:
Baixar

Coleções

Artigo de Periódico

Logo UFMG