Toxicity of carnoy's solution toward human keratinocytes: an in vitro study

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dc.creatorAecio Campos Pinto Junior
dc.creatorKarolina Skarlet Silva Viana
dc.creatorLucas Guimarães Abreu
dc.creatorClésia Cristina Nascentes
dc.creatorIvana Márcia Alves Diniz
dc.creatorRicardo Alves Mesquita
dc.creatorVanessa de Fátima Bernardes
dc.date.accessioned2025-08-04T18:44:23Z
dc.date.accessioned2025-09-09T01:17:39Z
dc.date.available2025-08-04T18:44:23Z
dc.date.issued2021
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1590/1807-3107bor-2021.vol35.0124
dc.identifier.issn1807-3107
dc.identifier.urihttps://hdl.handle.net/1843/84000
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofBrazilian Oral Research
dc.rightsAcesso Aberto
dc.subjectEspectrometria de massa
dc.subjectQuistos odontogênicos
dc.subject.otherHaCaT Cells
dc.subject.otherMass Spectrometry
dc.subject.otherOdontogenic Cysts
dc.titleToxicity of carnoy's solution toward human keratinocytes: an in vitro study
dc.typeArtigo de periódico
local.citation.spagee124
local.citation.volume35
local.description.resumoThe present study aimed to characterize the chemical elements and cytotoxicity of Carnoy's solution (CS) by comparing two different trademarked products (one Brazilian [NCS] and another imported [ICS]) using inductively coupled plasma mass spectrometry (ICP-MS) and human keratinocyte (HaCaT) cultures. For performing ICP-MS, the solutions were diluted according to calibration curves, and the chemical elements were analyzed with a spectrometer. HaCaT cells were exposed to CS concentrations ranging from 0.10% to 20% for 3 or 5 min. Cell viability was evaluated immediately (T0), 24 h (T1), and 7 days (T2) after exposure to CS using 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyl-tetrazolium bromide (MTT) reduction assay. Data were analyzed using a t-test for ICP-MS and analysis of variance followed by Tukey's post-hoc test for MTT assay, both considering statistical significance at p<0.05. ICP-MS results revealed that ICS presented significantly lower concentrations of 12 chemical elements than NCS. The results of MTT assay revealed that at T0, ICS was more cytotoxic than NCS regardless of the time of exposure (p < 0.05). At T1, the only difference between the groups was at a concentration of 0.10% after 5 min of exposure. At T2, at a concentration of 0.5%, ICS resulted in a significant reduction in cell viability compared to NCS (p < 0.05). Thus, the results showed that ICS was more cytotoxic than NCS. Collectively, our findings suggest that the individual compositions of different CS formulations should be investigated.
local.identifier.orcidhttps://orcid.org/0000-0002-2314-0140
local.identifier.orcidhttps://orcid.org/0000-0001-6467-1434
local.identifier.orcidhttps://orcid.org/0000-0003-2258-8071
local.identifier.orcidhttps://orcid.org/0000-0002-8514-442X
local.identifier.orcidhttps://orcid.org/0000-0003-4261-0037
local.identifier.orcidhttps://orcid.org/0000-0003-3207-4007
local.identifier.orcidhttps://orcid.org/0000-0003-0194-7434
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOPEDIATRIA E ORTODONTIA
local.publisher.departmentICB - DEPARTAMENTO DE PATOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.scielo.br/j/bor/a/LNRdJTLtFmbnKJWq7gP7WLM/?lang=en

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