Chromatographic bioanalysis of antiglaucoma drugs in ocular tissues

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dc.creatorPedro Henrique Reis da Silva
dc.creatorArmando da Silva Cunha Júnior
dc.creatorGerson Antônio Pianetti
dc.creatorChristian Fernandes
dc.date.accessioned2026-01-21T17:43:35Z
dc.date.issued2021-03-01
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.identifier.doihttps://doi.org/10.1016/j.jchromb.2020.122388
dc.identifier.issn1873-376X
dc.identifier.urihttps://hdl.handle.net/1843/1457
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Chromatography B
dc.rightsAcesso restrito
dc.subjectOlhos
dc.subjectMedicamentos
dc.subject.otherGlaucoma
dc.subject.otherPreparação da amostra
dc.subject.otherMétodos bioanalíticos
dc.subject.otherCromatografia líquida de alta eficiência
dc.subject.otherMedicamentos oftálmicos
dc.titleChromatographic bioanalysis of antiglaucoma drugs in ocular tissues
dc.typeArtigo de periódico
local.citation.epage16
local.citation.spage1
local.citation.volume1166
local.description.resumoGlaucoma is a heterogeneous group of multifactorial optic neuropathies and the leading cause of irreversible blindness and visual impairment. Epidemiological data has estimated that in 2020 there will be more than 80 million individuals affected by the disease worldwide. Nowadays, intraocular pressure (IOP) lowering is carried out mainly by pharmacotherapy, with different drugs. The study of ocular pharmacokinetics of antiglaucoma drugs, crucial for better understanding of ocular distribution, bioavailability, and pharmacodynamic parameters, can benefit the development of antiglaucoma drugs or formulations. Bioanalysis of drugs in ocular matrices is still underestimated, since it is challenging and rarely performed. Therefore, this review summarized the chromatographic methods employed for the quantification of several antiglaucoma drugs in different ocular matrices, discussing bioanalytical steps, such as sample preparation, separation, and detection. Animals and matrices as well as the challenges faced in ocular bioanalysis were also discussed. Ocular bioanalysis has been performed mainly in rabbits, the most adequate animal model for ocular studies. The matrix most used is aqueous humor, because it is cleaner and easier to sample. Sample preparation was carried out primarily employing classic techniques, such as liquid–liquid extraction, protein precipitation, and solid-phase extraction, with conventional solvents and sorbents. Chromatographic separation was achieved predominantly by reversed-phase liquid chromatography. Ultraviolet spectrophotometry and tandem mass spectrometry prevailed for detection, although other techniques, such as fluorimetry, have also been used. It was evidenced that more efforts must be directed towards miniaturized, eco-friendly, and non-terminal sampling for sample preparation. In its turn, ultra high-performance liquid chromatography and mass spectrometry should gain prominence in ocular bioanalysis for separation and detection, respectively, since it combines high separation capacity with selectivity and sensitivity, in addition to being an environmental friendly approach.
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS
local.publisher.initialsUFMG
local.subject.cnpqCIENCIAS DA SAUDE
local.url.externahttps://www.sciencedirect.com/science/article/pii/S1570023220312642

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