Photobiomodulation enhances cisplatin cytotoxicity in a culture model with oral cell lineages

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dc.creatorIvana Márcia Alves Diniz
dc.creatorGiovanna Ribeiro Souto
dc.creatorIuri Dornelas Prates Freitas
dc.creatorJosé Alcides Almeida de Arruda
dc.creatorJanine Mayra da Silva
dc.creatorTarcília Aparecida da Silva
dc.creatorRicardo Alves de Mesquita
dc.date.accessioned2025-04-28T14:07:55Z
dc.date.accessioned2025-09-08T22:49:22Z
dc.date.available2025-04-28T14:07:55Z
dc.date.issued2020
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1111/php.13152
dc.identifier.issn1751-1097
dc.identifier.urihttps://hdl.handle.net/1843/81922
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofPhotochemistry and photobiology
dc.rightsAcesso Restrito
dc.subjectLow-level light therapy
dc.subjectProteins
dc.subjectEnergy metabolism
dc.subjectKeratinocytes
dc.subjectCell survival
dc.subjectKi-67 antigen
dc.subjectTransforming growth factor beta1
dc.subjectEpidermal growth factor
dc.subjectReactive oxygen species
dc.subjectAdenosine triphosphate
dc.subjectApoptosis
dc.subjectCell death
dc.titlePhotobiomodulation enhances cisplatin cytotoxicity in a culture model with oral cell lineages
dc.typeArtigo de periódico
local.citation.epage190
local.citation.issue1
local.citation.spage182
local.citation.volume96
local.description.resumoCisplatin plays a central role in cancer chemotherapy, but resistance to this drug remains a major obstacle in treatment. Drawbacks related to cisplatin failure may be associated with cell energy metabolism. This study investigated whether photobiomodulation (PBM) can potentiate the effects of cisplatin on keratinocytes (HaCat) and cancer cells (SCC25 and HN12). Cells were treated with laser (GaAlAs; 660 nm; 60 mW; 0.33 J; 2.14 W cm-2 ; 11.7 J cm-2 and 6 s) and cisplatin (7.8 μg mL-1 ) to evaluate cell viability, Ki-67, VEGF, TGF-β1, EGF expression and ROS production. Observations were validated in the SCC25 cell lineage, where the type of cell death (necrosis/apoptosis) and the amount of ATP were assessed. Cell lineages showed increased sensitivity to cisplatin associated with PBM (Cis-PBM). Ki-67 was augmented in all cell lineages treated with Cis-PBM when compared to cisplatin alone (Cis). Cis or Cis-PBM significantly decreased VEGF expression in cancer cells, while no changes were seen in the expression of TGF-β1 or EGF compared to control. ROS levels were similar in the Cis and Cis-PBM groups. Cells treated with Cis-PBM died by apoptosis, leading to greater consumption of ATP. These observations suggest that PBM may potentiate the effects of cisplatin, leading to increased drug cytotoxicity and enhanced cell death.
local.identifier.orcidhttps://orcid.org/0000-0003-4261-0037
local.identifier.orcidhttps://orcid.org/0000-0003-3617-8794
local.identifier.orcidhttps://orcid.org/0000-0003-0918-0346
local.identifier.orcidhttps://orcid.org/0000-0002-6599-3950
local.identifier.orcidhttps://orcid.org/0000-0002-1473-7455
local.identifier.orcidhttps://orcid.org/0000-0001-9623-7835
local.identifier.orcidhttps://orcid.org/0000-0003-3207-4007
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/php.13152

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