Simultaneous saccharification isomerization and co-fermentation - SSICF: a new process concept for second-generation ethanol biorefineries combining immobilized recombinant enzymes and non-GMO saccharomyces

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dc.creatorEderson Pauloxavier Guilherme
dc.creatorLeticia Maria Zanphorlin
dc.creatorAmanda Silva de Sousa
dc.creatorRenan Yuji Miyamoto
dc.creatorCarlos Giovani Oliveira Bruziquesi
dc.creatorBruna Mara Aparecida de Carvalho Mesquita
dc.creatorSergio Henrique Sousa Santos
dc.creatorElizama Aguiar de Oliveira
dc.creatorJunio Cota
dc.date.accessioned2023-04-24T12:59:54Z
dc.date.accessioned2025-09-09T01:17:52Z
dc.date.available2023-04-24T12:59:54Z
dc.date.issued2021
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.identifier.doihttps://doi.org/10.1016/j.renene.2021.10.023
dc.identifier.issn0960-1481
dc.identifier.urihttps://hdl.handle.net/1843/52384
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofRenewable Energy
dc.rightsAcesso Restrito
dc.subjectÓxidos
dc.subjectHidróxidos
dc.subjectInibidores enzimáticos
dc.subjectBiomassa
dc.subjectFermentação
dc.subjectÁlcool
dc.titleSimultaneous saccharification isomerization and co-fermentation - SSICF: a new process concept for second-generation ethanol biorefineries combining immobilized recombinant enzymes and non-GMO saccharomyces
dc.typeArtigo de periódico
local.citation.epage284
local.citation.spage274
local.citation.volume182
local.description.resumoIntegrated bioprocess strategies may facilitate ethanol production from both C6 and C5 fractions of lignocellulosic feedstocks. We propose a new process concept, SSICF, where sugarcane bagasse is hydrolyzed simultaneously with xylose isomerization and the co-fermentation of C6 and C5 sugars. A commercial cocktail was supplemented with our multi-enzymatic system composed of three recombinant enzymes immobilized in Feroxyhyte magnetic nanoparticles: β-glucosidase, β-xylosidase and xylose isomerase. SSICF was performed using non-GMO Saccharomyces at pH 6.0 and 35 °C for 72 h in a synthetic medium containing cellobiose and xylose, and another medium containing pretreated sugarcane bagasse (PSB). The results of ethanol global yields in SSICF were 77.67% and 73.24% for the synthetic medium and PSB, respectively. In a nutshell, this is the first report of a successful proof-of-concept of SSICF with four rounds of enzyme recycling and a non-GMO yeast, an innovative process with high potential for industrial use.
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S0960148121014804

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