Patient-derived xenografts of a case of ameloblastic fibrodentinoma

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dc.creatorNubia Braga Pereira
dc.creatorCarolina Cavaliéri Gomes
dc.creatorJuliana C. de Souza
dc.creatorVictor Coutinho Bastos
dc.creatorFelipe Paiva Fonseca
dc.creatorGleide Fernandes de Avelar
dc.creatorWagner Henriques de Castro
dc.creatorAdriana Abalen Martins Dias
dc.creatorAdalberto Mosqueda-Taylor
dc.creatorRicardo Santiago Gomez
dc.date.accessioned2025-08-06T18:31:43Z
dc.date.accessioned2025-09-09T01:19:36Z
dc.date.available2025-08-06T18:31:43Z
dc.date.issued2019-05
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1111/odi.13056
dc.identifier.issn1601-0825
dc.identifier.urihttps://hdl.handle.net/1843/84117
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofOral Diseases
dc.rightsAcesso Restrito
dc.subjectNeoplasms
dc.subjectOdontogenic tumors
dc.subjectTransplantation, heterologous
dc.subjectKi-67 antigen
dc.subjectEvaluation study
dc.subjectPhosphorus
dc.subjectGenes
dc.subject.otherAmeloblastic fibrodentinoma
dc.subject.otherBenign tumors
dc.subject.otherMixed tumours
dc.subject.otherOdontogenic tumors
dc.subject.otherXenotransplantation
dc.titlePatient-derived xenografts of a case of ameloblastic fibrodentinoma
dc.typeArtigo de periódico
local.citation.epage1233
local.citation.issue4
local.citation.spage1229
local.citation.volume25
local.description.resumoObjectives: The establishment of animal models of xenotransplantation can contribute to the elucidation of the molecular pathogenesis of ameloblastic fibrodentinomas (AFD) and it also provides an opportunity for drug tests. We aimed to evaluate the possibility of AFD tumour growth in a patient-derived xenograft (PDX) model. In addition, we characterized the human tumour and the PDXs. Materials and methods: A sample of a recurrent AFD was obtained and two fragments were contralaterally implanted subcutaneously in an 8-week old female NUDE mouse. After 250 days, the PDXs were removed and submitted to histopathological and molecular analysis. Immunohistochemical reactions for Ki67 and the phosphorylated form of ERK1/2 were carried out in both, PDXs and human tumour, and the presence of BRAFV600E was assessed. Results: From day 135 onwards, the PDXs presented a growth peak and remained stable until day 250. Histopathologically, the PDXs presented the same features of the patient's tumour. Tumour cells exhibited Ki67 and pERK1/2 immunoexpression in the patient's tumour and PDX. The AFD was wild-type for BRAFV600E. Conclusion: The PDX model recapitulated well the human tumour after a long implantation time, representing a possible model to study the AFD and other odontogenic tumours pathobiology.
local.identifier.orcidhttps://orcid.org/0000-0001-9415-3240
local.identifier.orcidhttps://orcid.org/0000-0002-6714-3124
local.identifier.orcidhttps://orcid.org/0000-0003-1580-4995
local.identifier.orcidhttps://orcid.org/0000-0002-6657-4547
local.identifier.orcidhttps://orcid.org/0000-0002-2132-3218
local.identifier.orcidhttps://orcid.org/0000-0003-2745-2878
local.identifier.orcidhttps://orcid.org/0000-0001-8956-6016
local.identifier.orcidhttps://orcid.org/0000-0001-8770-8009
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE PATOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/odi.13056

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