DNA damage-related proteins in smokers and non-smokers with oral cancer

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dc.creatorLauren Frenzel Schuch
dc.creatorJosé Alcides Almeida de Arruda
dc.creatorMauro Henrique Nogueira Guimarães de Abreu
dc.creatorKarolina Skarlet Silva Viana
dc.creatorPatrícia Carlos Caldeira
dc.creatorMaria Cássia Ferreira Aguiar
dc.creatorVanessa Fátima Bernardes
dc.date.accessioned2023-11-14T20:34:47Z
dc.date.accessioned2025-09-09T00:57:37Z
dc.date.available2023-11-14T20:34:47Z
dc.date.issued2022-02-09
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1590/1807-3107bor-2022.vol36.0027
dc.identifier.issn18073107
dc.identifier.urihttps://hdl.handle.net/1843/60974
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofBrazilian Oral Research
dc.rightsAcesso Aberto
dc.subjectNeoplasias bucais
dc.subjectDano ao DNA
dc.subjectTabagismo
dc.subject.otherDNA damage
dc.subject.otherCheckpoint kinase 2
dc.subject.otherTumor suppressor protein p53
dc.subject.otherMouth neoplasms
dc.titleDNA damage-related proteins in smokers and non-smokers with oral cancer
dc.typeArtigo de periódico
local.citation.epage9
local.citation.spage1
local.citation.volume36
local.description.resumoTobacco smoking involves a high risk of human malignancies, including oral cancer, because it contains multiple carcinogens that cause genetic instability. Thus, a worse prognosis would be expected for cancer patients who are smokers. The aim of this study was to assess the DNA damage response through the expression of checkpoint kinase 2 (CHK2), H2A histone family member X (H2AX), and P53 among smokers and non-smokers with oral squamous cell carcinoma (OSCC). Associations between immunoexpression of proteins and clinicopathological data and histopathological grading were also analyzed. A total of 35 individuals (18 non-smokers and 17 smokers) with OSCC of the tongue and/or floor of the mouth were included. Immunohistochemistry for H2AX was conducted for the identification of double-strand breaks, CHK2, and P53 to evaluate the expression of this protein in cell cycle regulation. The sample consisted of 22 males and 13 females, with a mean age of 63.9±11.8 years. OSCC of non-smokers were well-differentiated tumors in 50% of the cases, and those of smokers were equally distributed into moderately differentiated and poorly differentiated tumors (35.3% each). Overall, 31 (88.6%) cases were CHK2-positive, 27 (77.1%) were H2AX-positive, and 23 (65.7%) were P53-positive, with no difference between smokers and non-smokers (p > 0.05). No association was found between proteins and clinicopathologic data (p > 0.05). Similarities in CHK2, H2AX, and P53 immunohistochemical staining patterns were observed between smokers and non-smokers, and immunoexpression was not associated with clinicopathological parameters. However, the findings indicated consistent expression of these proteins in OSCC.
local.identifier.orcidhttps://orcid.org/0000-0001-8794-5725
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA SOCIAL E PREVENTIVA
local.publisher.departmentICB - DEPARTAMENTO DE PATOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.scielo.br/j/bor/a/Hj5J5tHLyL4zfTMJyHzWGvD/?lang=en

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