Miniaturized methodologies for determining the total phenol and flavonoid concentrations and the antioxidant activity

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dc.creatorAne Patrícia Cacique
dc.creatorÉrica Soares Barbosa
dc.creatorGevany Paulino de Pinho
dc.creatorFlaviano Oliveira Silvério
dc.date.accessioned2023-03-28T11:11:28Z
dc.date.accessioned2025-09-09T00:05:20Z
dc.date.available2023-03-28T11:11:28Z
dc.date.issued2021-01-15
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.identifier.doihttps://doi.org/10.1007/s12161-020-01934-w
dc.identifier.issn1936-976X
dc.identifier.urihttps://hdl.handle.net/1843/51266
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofFood Analytical Methods
dc.rightsAcesso Restrito
dc.subjectFenóis
dc.subjectFlavonóides
dc.subjectEspectrofotometria
dc.titleMiniaturized methodologies for determining the total phenol and flavonoid concentrations and the antioxidant activity
dc.typeArtigo de periódico
local.citation.epage1120
local.citation.spage1110
local.citation.volume14
local.description.resumoThe present study describes the miniaturization of the conventional methodologies for determining the total phenol and flavonoid concentrations and the antioxidant activities using DPPH, ABTS, and ferric reducing antioxidant power (FRAP) methods. The amount of reagents used was reduced 25, 5.0, 3.6, 3.0, and 3.3 times relative to the conventional methodologies, representing a 67–96% reduction in the analysis costs. The miniaturized and conventional methodologies presented similar linearity in the same concentration range, with the determination and correlation coefficients greater than 0.99. The accuracy of the methodologies was evidenced by the relative standard deviations, which remained below 3%. The 38 samples comprising alcoholic beverages, teas, spices, jams, energy drinks, honey products, and grape derivatives analyzed using the two methodologies (miniaturized and conventional) presented similar average values. Therefore, the miniaturized methodology is proposed as an efficient, safe, and economical alternative to the conventional methods for determining the total phenol and flavonoid concentrations and the antioxidant activities using DPPH, ABTS, and FRAP methods in various matrices.
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://link.springer.com/article/10.1007/s12161-020-01934-w

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