GATA-1 mutation alters the spermatogonial phase and steroidogenesis in adult mouse testis

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dc.creatorMatheus Felipe Fonseca Gonçalves
dc.creatorVivian Vasconcelos Costa
dc.creatorAna Clara Campideli Santana
dc.creatorRoberta Araújo Lopes
dc.creatorRaphael Escorsim Szawka
dc.creatorGuilherme Mattos Jardim Costa
dc.creatorSamyra Maria dos Santos Nassif Lacerda
dc.creatorNathália de Lima e Martins Lara
dc.creatorCarolina Felipe Alves de Oliveira
dc.creatorAndré Felipe de Figueiredo Almeida
dc.creatorMarcos Rocha Gouvêa Brener
dc.creatorMarina Alcântara Cavalcante
dc.creatorAnderson Kenedy Santos
dc.creatorGabriel Henrique Campolina Silva
dc.date.accessioned2023-07-14T19:30:53Z
dc.date.accessioned2025-09-08T23:55:00Z
dc.date.available2023-07-14T19:30:53Z
dc.date.issued2021
dc.identifier.doihttps://doi.org/10.1016/j.mce.2021.111519
dc.identifier.issn0303-7207
dc.identifier.urihttps://hdl.handle.net/1843/56253
dc.languagepor
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofMolecular and Cellular Endocrinology
dc.rightsAcesso Aberto
dc.subjectMorfologia
dc.subjectLeydig, Celulas de
dc.subjectTesticulos
dc.subjectTestosterona
dc.subject.otherLeydig cell
dc.subject.otherTestis
dc.subject.otherΔdblGATA
dc.subject.otherTestosterone
dc.subject.otherGerm cell differentiation
dc.titleGATA-1 mutation alters the spermatogonial phase and steroidogenesis in adult mouse testis
dc.typeArtigo de periódico
local.citation.epage13
local.citation.spage1
local.citation.volume542
local.description.resumoGATA-1 is a transcription factor from the GATA family, which features zinc fingers for DNA binding. This protein was initially identified as a crucial regulator of blood cell differentiation, but it is currently known that the Gata-1 gene expression is not limited to this system. Although the testis is also a site of significant GATA-1 expression, its role in testicular cells remains considerably unexplored. In the present study, we evaluated the testicular morphophysiology of adult ΔdblGATA mice with a mutation in the GATA-1 protein. Regarding testicular histology, GATA-1 mutant mice exhibited few changes in the seminiferous tubules, particularly in germ cells. A high proportion of differentiated spermatogonia, an increased number of apoptotic pre-leptotene spermatocytes (Caspase-3-positive), and a high frequency of sperm head defects were observed in ΔdblGATA mice. The main differences were observed in the intertubular compartment, as ΔdblGATA mice showed several morphofunctional changes in Leydig cells. Reduced volume, increased number and down-regulation of steroidogenic enzymes were observed in ΔdblGATA Leydig cells. Moreover, the mutant animal showed lower serum testosterone concentration and high LH levels. These results are consistent with the phenotypic and biometric data of mutant mice, i.e., shorter anogenital index and reduced accessory sexual gland weight. In conclusion, our findings suggest that GATA-1 protein is an important factor for germ cell differentiation as well as for the steroidogenic activity in the testis.
local.identifier.orcidhttps://orcid.org/0000-0002-6657-4547
local.identifier.orcidhttps://orcid.org/0000-0002-0175-642X
local.identifier.orcidhttps://orcid.org/0000-0001-5225-4940
local.identifier.orcidhttps://orcid.org/0000-0002-2639-3469
local.identifier.orcidhttps://orcid.org/0000-0003-4577-6243
local.identifier.orcidhttps://orcid.org/0000-0002-8170-6384
local.identifier.orcidhttps://orcid.org/0000-0002-8605-8574
local.identifier.orcidhttps://orcid.org/0000-0002-6185-3491
local.identifier.orcidhttps://orcid.org/0000-0002-4473-3340
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S0303720721003634?via%3Dihub

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