Degradation of sugarcane bagasse by Cockroach Consortium bacteria

Página do item simplificado
dc.creatorDanielle Bruno de Carvalho
dc.creatorDouglas Antonio Alvaredo Paixão
dc.creatorGabriela Felix Persinoti
dc.creatorJúnio Cota Silva
dc.creatorSarita Cândida Rabelo
dc.creatorAdriana Grandis
dc.creatorAmanda Pereira de Souza
dc.creatorMarcos Silveira Buckeridge
dc.creatorRoberto Ruller
dc.creatorLeticia Maria Zanphorlin
dc.creatorFábio Márcio Squina
dc.creatorJoão Paulo Lourenço Franco Cairo
dc.creatorEdnildo de Alcântara Machado
dc.date.accessioned2024-02-20T12:09:13Z
dc.date.accessioned2025-09-08T23:01:30Z
dc.date.available2024-02-20T12:09:13Z
dc.date.issued2022-01-13
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipFAPERJ - Fundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de Janeiro
dc.description.sponsorshipFAPESP - Fundação de Amparo à Pesquisa do Estado de São Paulo
dc.description.sponsorshipOutra Agência
dc.identifier.doihttps://doi.org/10.1007/s12155-021-10363-4
dc.identifier.issn1939-1242
dc.identifier.urihttps://hdl.handle.net/1843/64303
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofBioEnergy Research
dc.rightsAcesso Restrito
dc.subjectBiomassa
dc.subjectLignina - Biodegradação
dc.subjectCelulose
dc.subjectAnaerobiose
dc.titleDegradation of sugarcane bagasse by Cockroach Consortium bacteria
dc.typeArtigo de periódico
local.citation.epage1156
local.citation.spage1144
local.citation.volume15
local.description.resumoThe development of new lignocellulosic enzyme cocktails to digest raw material for the successful production of biofuels still represents great economic and scientific challenges. The aim of this study was the characterization of an aerobic consortium derived from the gut of the cockroach Nauphoeta cinerea (NaLC) under morphological, chemical, biochemical, and genetic views. Initially, the assembly of the consortium started by dissection and incubation of hindgut of N cinerea with medium containing sugarcane bagasse (BED) as carbon source. After 1 week of cultivation, the NaLC degraded about 55% of lignocellulosic material (mainly cellulose) and also promoted a significant decreased in the BED length. The biochemical analysis of consortium supernatants showed activities against Avicel, β-glucan, xylan, and CMC. The metagenomics analysis of 16S rDNA from NaLC showed 22 bacterial genomes with a predominance of Bacteroidetes (50.6%) and Proteobacteria (47.7%) with Flavobacterium spp. and Sphingomonas spp., respectively. The shotgun metagenomic analysis revealed the presence of several CAZymes, such as cellulases, hemicellulases, carbohydrate esterases, and enzymes with auxiliary activities. During the shotgun metagenomic analysis, various CAZymes organized as polysaccharide utilization loci (PUL) from Niabela sp. bacteria in NaLC were identified. It was also observed a new locus named here as LUL (lignin utilization locus) containing many genes as oxidases, dehydrogenases, peroxidases, and others that could be involved in lignin metabolization. So, this study described under different approaches an unprecedented aerobic consortium could be a useful resource of novel genes and enzymes involved in degradation of sugarcane bagasse.
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://link.springer.com/article/10.1007/s12155-021-10363-4

Arquivos

Licença do pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
License.txt
Tamanho:
1.99 KB
Formato:
Plain Text
Descrição:
Baixar

Coleções

Artigo de Periódico

Logo UFMG