Unveiling 3D physicochemical changes of sugarcane bagasse during sequential acid/alkali pretreatments by synchrotron phase-contrast imaging

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dc.creatorAugusta Cerceau Isaac Neta
dc.creatorSilvio Silvério da Silva
dc.creatorFelipe Antonio Fernandes Antunes
dc.creatorRodolfo Conti Barreto
dc.creatorLuciano Andrey Montoro
dc.creatorÂngelo Malachias de Souza
dc.creatorPatrícia Massara Martinelli
dc.creatorGregory Thomas Kitten
dc.creatorHenning Markötter
dc.creatorIngo Manke
dc.date.accessioned2023-05-22T16:12:16Z
dc.date.accessioned2025-09-08T23:30:29Z
dc.date.available2023-05-22T16:12:16Z
dc.date.issued2018-02-16
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipFAPESP - Fundação de Amparo à Pesquisa do Estado de São Paulo
dc.identifier.doihttps://doi.org/10.1016/j.indcrop.2018.01.028
dc.identifier.issn1872-633X
dc.identifier.urihttps://hdl.handle.net/1843/53740
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofIndustrial Crops and Products
dc.rightsAcesso Restrito
dc.subjectLignina
dc.subjectBiocombustíveis
dc.subjectRadiação sincrotrônica
dc.subjectBagaço de cana
dc.subject.otherLignin
dc.subject.otherBiofuel
dc.subject.otherPhase-contrast tomography
dc.subject.otherSynchrotron radiation
dc.subject.otherSugarcane bagasse
dc.titleUnveiling 3D physicochemical changes of sugarcane bagasse during sequential acid/alkali pretreatments by synchrotron phase-contrast imaging
dc.typeArtigo de periódico
local.citation.epage27
local.citation.spage19
local.citation.volume114
local.description.resumoSeveral pretreatment strategies focus on the removal of a significant part of lignin from plant cell walls, as it is considered the main barrier to the process of deconstructing biomass to simple sugars by hydrolytic enzymes. The ability to chemically differentiate and spatially locate lignins across cell walls provides an important contribution to the effort to improve these processes. Here, we present a novel approach using synchrotron phase-contrast tomography to probe the physicochemical features of plant cell walls. In addition to the 3D cellular architecture, the distribution of dense packed lignin–carbohydrate complexes (or, simply dense lignin) over larger regions and within cell walls has been successfully provided. We examined in particular the effect of the sequential H2SO4 and NaOH pretreatment on sugacane bagasse. Our results revealed that aggregates of dense lignin form elongated 3D structures in cell corners (CC) following the orientation of the sclerenchyma fibers; a remarkable result in the light of the conventional perception that lignin particles are discrete and roughly spherical. After the acid pretreatment, a considerable fraction of the dense lignin primarily located in CCs was removed; and it was further dissolved with the subsequent alkali treatment. Unexpectedly, the two-step pretreatment did not contribute to the cellulose accessibility in terms of available surface area and porosity. This study provides new insights into the underlying mechanisms of biomass deconstruction by pretreatments; and the approach established here can be extended to other systems relevant to the bioenergy and biotechnology arenas.
local.identifier.orcidhttps://orcid.org/0000-0001-8617-0956
local.identifier.orcidhttps://orcid.org/0000-0003-0669-2784
local.identifier.orcidhttps://orcid.org/0000-0002-5157-3971
local.identifier.orcidhttps://orcid.org/0000-0002-8703-4283
local.identifier.orcidhttps://orcid.org/0000-0002-4044-9932
local.identifier.orcidhttps://orcid.org/0000-0003-3464-6793
local.identifier.orcidhttps://orcid.org/0000-0001-9795-5345
local.publisher.countryBrasil
local.publisher.departmentENG - DEPARTAMENTO DE ENGENHARIA METALÚRGICA
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.departmentICX - DEPARTAMENTO DE FÍSICA
local.publisher.departmentICX - DEPARTAMENTO DE QUÍMICA
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S0926669018300281

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