Reproducibility, repeatability and level of difficulty of two methods for tumor budding evaluation in oral squamous cell carcinoma

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dc.creatorPriscila Laiza Rubim Leão
dc.creatorHelvécio Marangon Junior
dc.creatorVictória Vasconcellos Moreira Melo
dc.creatorÂngela Braga Caixeta
dc.creatorPaulo Eduardo Alencar Souza
dc.creatorMaria Cassia Ferreira de Aguiar
dc.creatorMartinho Campolina Rebello Horta
dc.date.accessioned2024-08-02T19:26:43Z
dc.date.accessioned2025-09-09T01:03:01Z
dc.date.available2024-08-02T19:26:43Z
dc.date.issued2017
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1111/jop.12578
dc.identifier.issn1600-0714
dc.identifier.urihttps://hdl.handle.net/1843/72460
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Oral Pathology & Medicine
dc.rightsAcesso Restrito
dc.subjectMouth neoplasms
dc.subjectSquamous cell carcinoma of head and neck
dc.subjectColor
dc.subjectImmune system
dc.subjectHematoxylin
dc.subjectKeratins
dc.subjectReproducibility of results
dc.subjectEvaluation study
dc.subjectReplica techniques
dc.subject.otheroral squamous cell carcinoma
dc.subject.otherbudding
dc.titleReproducibility, repeatability and level of difficulty of two methods for tumor budding evaluation in oral squamous cell carcinoma
dc.typeArtigo de periódico
local.citation.epage955
local.citation.issue10
local.citation.spage949
local.citation.volume46 Journal of oral pathology & medicine
local.description.resumoBackground: This study aimed to analyze the reproducibility, repeatability, and level of difficulty of two methods for tumor budding evaluation in oral squamous cell carcinoma (OSCC): staining by hematoxylin and eosin (HE) and immunostaining for multicytokeratin. Methods: The evaluation of tumor budding was performed by three examiners in 103 samples of OSCC, using the two methods. A Likert-type scale was used to measure the difficulty in the assessment. The interexaminer agreement (reproducibility) was estimated using Fleiss's kappa and the intra-examiner agreement (repeatability) was estimated using Cohen's kappa. The agreement between the two methods was evaluated using Cohen's Kappa. The Friedman test was used to compare the three examiners' perceived levels of difficulty of assessment. The Wilcoxon test was used to compare the level of difficulty of the evaluation between the two methods. Results: Reproducibility by the immunostaining method for multicytokeratin was substantial, being higher than the only fair agreement by the HE. Repeatability by the HE ranged from moderate to substantial among examiners, regardless of the examiner's experience. Repeatability by the immunostaining method for multicytokeratin did not vary among examiners, showing almost perfect agreement. The agreement between the two methods ranged from fair to moderate among examiners, being lower in the less experienced examiner. All the examiners presented greater difficulty in the evaluation by the HE. Conclusion: In view of the unsatisfactory agreement between the two methods of tumor budding evaluation in OSCC, it is recommended that this evaluation should be performed by the immunostaining method for multicytokeratin, considering its higher reproducibility, greater replicability, and lower difficulty compared to the HE.
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/jop.12578

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