Immunomodulatory and neuroprotective effect of cryopreserved allogeneic mesenchymal stem cells on spinal cord injury in rats

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dc.creatorIsabel Rodrigues Rosado
dc.creatorAlfredo de Miranda Goes
dc.creatorEliane Goncalves de Melo
dc.creatorPablo Carvalho
dc.creatorE. G. L. Alves
dc.creatorT. M. Tagushi
dc.creatorJ. L. Carvalho
dc.creatorJuneo de Freitas Silva
dc.creatorM. S. L. Lavor
dc.creatorK. M. Oliveira
dc.creatorRogéria Serakides
dc.date.accessioned2025-03-06T19:54:52Z
dc.date.accessioned2025-09-09T01:07:57Z
dc.date.available2025-03-06T19:54:52Z
dc.date.issued2017-03-22
dc.format.mimetypepdf
dc.identifier.issn1676-5680
dc.identifier.urihttps://hdl.handle.net/1843/80541
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofGenetics and Molecular Research
dc.rightsAcesso Aberto
dc.subjectNeurologia
dc.subjectInflamação
dc.subjectMedicina veterinária
dc.subjectMedicina
dc.subjectTerapia celular
dc.subject.otherNeurology
dc.subject.otherCell therapy
dc.subject.otherRegenerative medicine
dc.subject.otherInflammation
dc.titleImmunomodulatory and neuroprotective effect of cryopreserved allogeneic mesenchymal stem cells on spinal cord injury in rats
dc.typeArtigo de periódico
local.citation.epage24
local.citation.issue1
local.citation.spage1
local.citation.volume16
local.description.resumoThis study aimed to evaluate the immunomodulatory and neuroprotective effects of allogeneic and cryopreserved mesenchymal stem cells (MSCs) on spinal cord injury. A total of 120 rats were distributed into the following groups: negative control (NC) - without injury, positive control (PC) - with injury without treatment, and group treated with MSC (GMSC) - with injury and treated. Motor function was evaluated by the BBB test at 24, 48, and 72 h and at 8 and 21 postoperative days. Spinal cords were evaluated by histopathology and immunohistochemistry to determine the expression of CD68, NeuN, and GFAP. IL-10, TNF-α, IL-1β, TGF-β, BDNF, GDNF, and VEGF expression was quantified by RT-PCR. The GMSC presented higher scores for motor function at 72 h and 8 and 21 days after injury, lower expression of CD68 at 8 days, and lower expression of GFAP at 21 days compared to the PC. In addition, higher expression of NeuN and lower degeneration of the white matter occurred at 21 days. The GMSC also showed higher expression of IL-10 24 h after injury, GDNF at 48 h and 8 days, and VEGF at 21 days. Moreover, lower expression of TNF-α was observed at 8 and 21 days and TGF-β at 24 h and 21 days. There were no differences in the expression of IL-1β and BDNF between the GMSC and PC. Thus, cryopreserved MSCs promote immunomodulatory and neuroprotective effects in rats with spinal cord injury by increasing IL-10, GDNF, and VEGF expression and reducing TNF-α and TGF-β expression.
local.identifier.orcidhttps://orcid.org/0000-0001-7819-4253
local.identifier.orcidhttps://orcid.org/0000-0002-0262-949X
local.identifier.orcidhttps://orcid.org/0000-0002-9881-1236
local.identifier.orcidhttps://orcid.org/0000-0001-5374-6242
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
local.publisher.departmentVETER - ESCOLA DE VETERINARIA
local.publisher.initialsUFMG
local.url.externahttps://www.funpecrp.com.br/gmr/year2017/vol16-1/pdf/gmr-16-01-gmr.16019555.pdf

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