The Effect of Norepinephrine and Caffeic Acid on Cytokines and Nitric Oxide Production by Granulocytes is Age-Dependent

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There is a large increase in the number of elderly people in modern societies. This demographic phenomenon has been paralleled by an epidemic of chronic diseases and inflammatory process usually associated with advanced age. In this work we seek to fill a gap that still exists in the process of immunoregulation “old guy’ looking to narrow the knowledge regarding their correlation with the immune system and the nervous system. The Nitric Oxide (NO) and Interleukins productions were studied in healthy subjects in age ranging from 20 to 80 years divided in three age groups: (20-39), (40-59) and (60-80) years. NO production was quantified in a Griess reaction. The results were expressed through the nitrite concentration calculated by linear regression using a standard curve obtained from a solution of sodium nitrite and 1mM RPMI. Interleukins 8, 10 and 4 (IL-8, IL-10 and IL-4) productions were measured by sandwich ELISA. Ours results demonstrated that Norepinephrine (NoAD) can no longer modulate the inflammatory cytokine profile and that there is a significant decay of the contribution of Caffeic Acid (CA) as an antioxidant and / or anti-inflammatory age-related. Thus, our results showed a lack of NoAD and CA modulation in human granulocyte in ageing dependent process.

Abstract

Assunto

Envelhecimento, Granulócitos, Ácidos Cafeicos, Citocinas

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Aging, Granulocyte, Caffeic acids, Nitric oxid, Cytokines

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https://researchopenworld.com/the-effect-of-norepinephrine-and-caffeic-acid-on-cytokines-and-nitric-oxide-production-by-granulocytes-is-age-dependent/

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