Research Note: Antimicrobial resistance profile of Enterococcus spp. isolated from the eggshell of laying hens submitted to pharmacological treatment
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Universidade Federal de Minas Gerais
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Artigo de periódico
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Membros da banca
Resumo
The occurrence of antimicrobial resis tance in microorganisms isolated from eggshells, espe cially Enterococcus, might serve as a parameter to
assess the selection of bacteria due to the use of drugs in
the diet of laying hens. In order to evaluate the fre quency and the antimicrobial resistance profile of
Enterococcus spp. isolated from the eggshells, 225 Hy line laying hens were submitted to a 25-d long trial. The
treatments were the following: hens fed ration without
antimicrobials (control) and groups that received oxy tetracycline (10 mg kg 1
), doxycycline (20 mg kg 1
), lin comycin (50 mg kg 1
), and enrofloxacin (10 mg kg 1
) in
the ration for 5 d. Six replications were analyzed per
treatment, composed of a pool of 5 eggs each. They were
collected before treatment and on days 3, 6, 15, and 25,
totaling 150 samples. Eggshells were submitted to
counts of Enterococcus spp., which were identified by
proteomic analyses. Antimicrobial resistance was deter mined by the disk-diffusion test. It was observed that
97.3% (n = 146) of the samples were contaminated with
Enterococcus spp. There were no differences (P > 0.05)
in the bacterial counts between treatments on the
same day of evaluation. E. faecalis and E. faecium
were the most frequent on the eggshells of all treat ments. Multiresistance to the four classes of antimicro bials was also verified in the isolated bacteria. A total
of 83% of the Enterococcus isolates showed resistance
to neomycin, which was not administered to the hens,
demonstrating an environmental problem. Thus, feed ing laying hens with diets added with antimicrobials
induces drug resistance in Enterococcus spp., isolated
form the eggshells.
Abstract
Assunto
Medicina Veterinária, Ovos, Produção animal
Palavras-chave
Eggshell, Microbiological quality, Multiresistance, Enterococcus, MALDI-TOF/MS
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Endereço externo
https://www.sciencedirect.com/science/article/pii/S0032579121006271?via%3Dihub