Perfurações experimentais de furca tratadas com MTA acrescido de selênio: análise da resposta imune

Abstract

Furcal perforation is a mechanical or pathological communication between the root canal system and outer surface of the tooth. It may allow bacterial contamination and subsequent periodontal tissue inflammation and loss of supportive tissue. Currently, the MTA (mineral trioxide aggregate) is the most suitable material in the treatment of these perforations to be biocompatible and provide good sealing. The literature reports the selenium's ability to inhibit bacterial growth, alter bone metabolism and stimulate the immune system. The objective of this study was to evaluate the immune response of MTA associated with selenium. In this study, experimental furcal perforations were induced in molar of germ-free mice. The first maxillary left molar had the furcal perforated and treated with MTA / Selenium (experimental group) and the right molar, furcal was perforated and treated with MTA (control group). The animals were killed in 07, 14 and 21 days after the intervention (n = 5). mRNA was extracted from periradicular tissues and the eexpressions of TGF-β, TNF-α, IFN γ, IL10, IL-4, RANK, RANKL, IL-1α, IL-17A e HPRT were investigated by real time PCR. In the experimental groups, on 21 days post MTA+Se sealing it was observed an upregulation of the mRNA levels of TNF-α and IL-10 compared with the control group (p<0.05). Assessment revealed basal expression of IL-1α, IFN-γ, RANK, RANKL, IL17A,IL-4 and TGF-β gene expression all long of the experimental times, in both groups treated with MTA or MTA+Se (p>0.05). The MTA+Se sealing favored the expression of TNF-α and IL-10 in the later phase of response (21th day). The increase of these cytokines was probably due to immunoregulation of Se levels