Thrombin generation in vivo and ex vivo in sickle cell disease patients

Página do item simplificado
dc.creatorValéria Sutana Ladeira
dc.creatorSílvia Letícia de Oliveira Toledo
dc.creatorLetícia Gonçalves Resende Ferreira
dc.creatorMarina Mendes Oliveira
dc.creatorAna Paula Ferreira Silva
dc.creatorWander Valadares de Oliveira Jr
dc.creatorRita Carolina Figueiredo Duarte
dc.creatorCristiane de Oliveira Renó
dc.creatorLuci Maria Sant'ana Dusse
dc.creatorHérica Lima dos Santos
dc.creatorMaria das Graças Carvalho
dc.creatorMelina de Barros Pinheiro
dc.creatorDanyelle Romana Alves Rios
dc.date.accessioned2024-11-26T23:57:30Z
dc.date.accessioned2025-09-09T00:50:15Z
dc.date.available2024-11-26T23:57:30Z
dc.date.issued2021-01
dc.identifier.doi10.1016/j.thromres.2020.10.040
dc.identifier.issn0049-3848
dc.identifier.urihttps://hdl.handle.net/1843/78304
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofThrombosis Research
dc.rightsAcesso Restrito
dc.subjectDoença falciforme
dc.subject.otherDoença falciforme
dc.subject.otherGeração de trombina
dc.subject.otherHipercoagulabilidade
dc.titleThrombin generation in vivo and ex vivo in sickle cell disease patients
dc.typeArtigo de periódico
local.citation.epage171
local.citation.spage165
local.citation.volume197
local.description.resumoAbstract Activation of coagulation is an important hallmark of sickle cell disease (SCD) and it is believed that hypercoagulability plays a role to the disease pathophysiology. Studies have sought to identify how hemostatic biomarkers are expressed in SCD, however, the results are inconclusive. In this context, our objective was to evaluate the thrombin generation in vivo and ex vivo in SCD patients and the association between these biomarkers and the use of HU. This cross-sectional study was carried out with patients diagnosed with SCD, users or not of Hydroxyurea (HU), and healthy individuals as controls. D dimer (D-Di) was evaluated by ELISA and (TGT) thrombin generation test by CAT method. D-Di plasma levels were significantly higher in SCD patients when compared to the controls. TGT parameters such as peak, ETP and normalized ETP at low TF concentration and time-to-peak, peak, ETP and normalized ETP values at high TF concentration were lower in SCD patients than in controls. In contrast, the normalized activated protein C sensitivity ratio (nAPCsr) was higher in patients compared to controls, indicating resistance to the action of this natural anticoagulant. Regarding the use of HU, comparing users and non-users of this drug, no difference was observed in D-Di levels and in most TGT parameters. Our data analyzed together allow us to conclude that patients with SCD present a state of hypercoagulability in vivo due to the higher levels of D-Di and resistance to APC assessed ex vivo which is consistent with the coagulation imbalance described in SCD patients.
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S0049384820305995

Arquivos

Licença do pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
License.txt
Tamanho:
1.99 KB
Formato:
Plain Text
Descrição:
Baixar

Coleções

Artigo de Periódico

Logo UFMG