Influence of ACE I/D polymorphism on circulating levels of plasminogen activator inhibitor 1, D-dimer, ultrasensitive C-reactive protein and transforming growth factor β1 in patients undergoing hemodialysis

Página do item simplificado
dc.creatorSara Santos de Carvalho
dc.creatorAna Cristina Simões e Silva
dc.creatorAdriano de Paula Sabino
dc.creatorFernanda Cristina Gontijo Evangelista
dc.creatorKarina Braga Gomes
dc.creatorLuci Maria SantAna Dusse
dc.creatorDanyelle Romana Alves Rios
dc.date.accessioned2022-03-09T18:45:50Z
dc.date.accessioned2025-09-09T00:44:50Z
dc.date.available2022-03-09T18:45:50Z
dc.date.issued2016-03-29
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1371/journal.pone.0150613
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/1843/39901
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofPlos One
dc.rightsAcesso Aberto
dc.subjectDiálise
dc.subjectPlasma
dc.subjectDoenças cardiovasculares
dc.subjectInsuficiência renal crônica
dc.subjectReação em cadeia da polimerase
dc.subjectGenótipo
dc.subjectAlelos
dc.titleInfluence of ACE I/D polymorphism on circulating levels of plasminogen activator inhibitor 1, D-dimer, ultrasensitive C-reactive protein and transforming growth factor β1 in patients undergoing hemodialysis
dc.typeArtigo de periódico
local.citation.epage11
local.citation.issue3
local.citation.spage1
local.citation.volume11
local.description.resumoBackground: There is substantial evidence that chronic renal and cardiovascular diseases are associated with coagulation disorders, endothelial dysfunction, inflammation and fibrosis. Angiotensin-Converting Enzyme Insertion/Deletion polymorphism (ACE I/D polymorphism) has also be linked to cardiovascular diseases. Therefore, this study aimed to compare plasma levels of ultrassensible C-reactive protein (usCRP), PAI-1, D-dimer and TGF-β1 in patients undergoing HD with different ACE I/D polymorphisms. Methods: The study was performed in 138 patients at ESRD under hemodialysis therapy for more than six months. The patients were divided into three groups according to the genotype. Genomic DNA was extracted from blood cells (leukocytes). ACE I/D polymorphism was investigated by single polymerase chain reaction (PCR). Plasma levels of D-dimer, PAI-1 and TGF-β1 were measured by enzyme-linked immunosorbent assay (ELISA), and the determination of plasma levels of usCRP was performed by immunonephelometry. Data were analyzed by the software SigmaStat 2.03. Results: Clinical characteristics were similar in patients with these three ACE I/D polymorphisms, except for interdialytic weight gain. I allele could be associated with higher interdialytic weight gain (P =0.017). Patients genotypedas DDandasIDhadsignificantly higher levels of PAI-1 than those with II genotype. Other laboratory parameters did not significantly differ among the three subgroups (P = 0.033). Despite not reaching statistical significance, plasma levels of usCRPwerehigherinpatients carrying the D allele. Conclusion: ACEI/Dpolymorphisms could beassociated with changes in the regulation of sodium, fibrinolytic system, and possibly, inflammation. Our data showed that high levels of PAI-1 are detected when Dallele is present, whereas greater interdialytic gain is associated with the presence of I allele. However, further studies with different experimental designs are necessary to elucidate the mechanisms involved in these associations.
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS
local.publisher.departmentMED - DEPARTAMENTO DE PEDIATRIA
local.publisher.initialsUFMG
local.url.externahttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0150613

Arquivos

Pacote original

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
Influence of ACE I-D polymorphism on circulating levels of plasminogen activator inhibitor 1, D-dimer, ultrasensitive C-reactive protein and transforming growth factor β1 in patients undergoing.PDF
Tamanho:
419.01 KB
Formato:
Adobe Portable Document Format
Baixar

Licença do pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
License.txt
Tamanho:
1.99 KB
Formato:
Plain Text
Descrição:
Baixar

Coleções

Artigo de Periódico

Logo UFMG