Immunophenotype of neutrophils in oral squamous cell carcinoma patients

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dc.creatorPatrícia Carlos Caldeira
dc.creatorÉrica Leandro Marciano Vieira
dc.creator. Alexandre de Andrade Sousa
dc.creatorAntonio Lucio Teixeira Junior
dc.creatorMaria Cássia Ferreira de Aguiar
dc.date.accessioned2025-06-12T20:37:53Z
dc.date.accessioned2025-09-08T23:08:49Z
dc.date.available2025-06-12T20:37:53Z
dc.date.issued2017-10
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1111/jop.12575
dc.identifier.issn1600-0714
dc.identifier.urihttps://hdl.handle.net/1843/82928
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Oral Pathology & Medicine
dc.rightsAcesso Restrito
dc.subjectImmunohistochemistry
dc.subjectEnzyme-linked immunosorbent assay
dc.subjectFlow cytometry
dc.subjectNeutrophils
dc.subjectCarcinoma, squamous cell
dc.subjectProteins
dc.subjectTumor necrosis factors
dc.subjectImmunophenotyping
dc.subjectT-lymphocytes
dc.subjectCytokines
dc.subject.otherELISA
dc.subject.otherFlow cytometry
dc.subject.otherImmunohistochemistry
dc.subject.otherNeutrophils
dc.subject.otherOutcome
dc.subject.otherSquamous cell carcinoma
dc.titleImmunophenotype of neutrophils in oral squamous cell carcinoma patients
dc.typeArtigo de periódico
local.citation.epage709
local.citation.issue9
local.citation.spage703
local.citation.volume46
local.description.resumoBackground: The aim was to explore the immunophenotype of neutrophils and lymphocytes and the inflammatory mediators in patients with oral squamous cell carcinoma, comparing with controls; and to associate with clinicopathological data. Methods: Blood was collected from 13 patients and 13 controls. The immunophenotype of neutrophils (CD66b, CD16, CD11a, arginase-1), T lymphocytes (CD4, CD8) and the intracellular cytokine production (IL-10, TNF, IFN-γ) was evaluated by flow cytometry. Plasma concentration of sVCAM-1, sTNF-RI, sTNF-RII, and IL-1β was measured by ELISA. MPO, Lipocalin-2/NGAL, sICAM-1, and p-selectin were quantified by Luminex assay. The excised tumors were submitted to immunohistochemistry for neutrophils (CD66b) and lymphocytes (CD3, CD4, CD8). Association with clinical data was explored. P values <.05 were considered significant. Results: Patients presented higher percentage of neutrophils and lower lymphocytes, resulting a higher neutrophil/lymphocyte ratio than controls. They also presented higher percentage of neutrophils expressing CD66b+ , CD66b+ Arginase-1+ , CD66b+ IL10+ , CD66b+ TNF+ , CD66b+ Arginase-1+ IL-10+ , and lower CD66b+ CD16+ CD11a+ and CD66b+ Arginase-1+ TNF+ . CD66b+ neutrophils were detected in all tumors, with a CD66b+ /CD3+ ratio of 0.40. Patients showed higher concentration of plasmatic sVCAM-1 and lower Lipocalin-2/NGAL. Patients with good outcome presented lower percentage of neutrophils, higher percentage of lymphocytes, and lower NLR than patients who died. Conclusion: The amount and immunophenotype of neutrophils and lymphocytes differ between patients and healthy individuals, with a pro-tumorigenic profile of neutrophils. As these cells also get within tumor microenvironment, they possibly exert systemic and local functions in cancer pathogenesis. The association of neutrophil count with outcome corroborates recent studies and this merits further investigation for applicability as a prognosticator.
local.identifier.orcidhttps://orcid.org/0000-0002-9179-0145
local.identifier.orcidhttps://orcid.org/0000-0002-4147-5614
local.identifier.orcidhttps://orcid.org/0000-0002-4204-9334
local.identifier.orcidhttps://orcid.org/0000-0002-9621-5422
local.identifier.orcidhttps://orcid.org/0000-0001-5134-3466
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.departmentMED - DEPARTAMENTO DE CIRURGIA
local.publisher.departmentMED - DEPARTAMENTO DE CLÍNICA MÉDICA
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/jop.12575

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