High genomic variability in equine infectious anemia virus obtained from naturally infected horses in Pantanal, Brazil: an endemic region case

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dc.creatorCamila Dantas Malossi
dc.creatorEduardo Gorzoni Fioratti
dc.creatorJedson Ferreira Cardoso
dc.creatorAngelo Jose Magro
dc.creatorErna Geessien Kroon
dc.creatorDaniel Moura de Aguiar
dc.creatorAlice Mamede Costa Marque Borges
dc.creatorMarcia Furlan Nogueira
dc.creatorLeila Sabrina Ullmann
dc.creatorJoão Pessoa Araújo Júnior
dc.date.accessioned2025-02-11T21:07:42Z
dc.date.accessioned2025-09-08T23:56:39Z
dc.date.available2025-02-11T21:07:42Z
dc.date.issued2020-02-12
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.3390/v12020207
dc.identifier.issn1999-4915
dc.identifier.urihttps://hdl.handle.net/1843/79928
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofViruses
dc.rightsAcesso Aberto
dc.subjectAnemia Infecciosa Equina
dc.subjectSequenciamento Completo do Genoma
dc.subjectAnemia
dc.subjectAnimais - Doenças
dc.subjectCavalos - Doenças
dc.subject.otherEquine infectious anemia virus
dc.subject.otherMolecular characterization
dc.subject.otherEndemic region
dc.subject.otherRNA genome
dc.subject.otherEquine
dc.titleHigh genomic variability in equine infectious anemia virus obtained from naturally infected horses in Pantanal, Brazil: an endemic region case
dc.typeArtigo de periódico
local.citation.epage15
local.citation.issue2
local.citation.spage1
local.citation.volume12
local.description.resumoEquine infectious anemia virus (EIAV) is a persistent lentivirus that causes equine infectious anemia (EIA). In Brazil, EIAV is endemic in the Pantanal region, and euthanasia is not mandatory in this area. All of the complete genomic sequences from field viruses are from North America, Asia, and Europe, and only proviral genomic sequences are available. Sequences from Brazilian EIAV are currently available only for gag and LTR regions. Thus, the present study aimed for the first time to sequence the entire EIAV genomic RNA in naturally infected horses from an endemic area in Brazil. RNA in plasma from naturally infected horses was used for next-generation sequencing (NGS), and gaps were filled using Sanger sequencing methodology. Complete viral genomes of EIAV from two horses were obtained and annotated (Access Number: MN560970 and MN560971). Putative genes were analyzed and compared with previously described genes, showing conservation in gag and pol genes and high variations in LTR and env sequences. Amino acid changes were identified in the p26 protein, one of the most common targets used for diagnosis, and p26 molecular modelling showed surface amino acid alterations in some epitopes. Brazilian genome sequences presented 88.6% nucleotide identity with one another and 75.8 to 77.3% with main field strains, such as EIAV Liaoning, Wyoming, Ireland, and Italy isolates. Furthermore, phylogenetic analysis suggested that this Brazilian strain comprises a separate monophyletic group. These results may help to better characterize EIAV and to overcome the challenges of diagnosing and controlling EIA in endemic regions.
local.identifier.orcidhttps://orcid.org/0000-0002-3841-2479
local.identifier.orcidhttps://orcid.org/0000-0002-1982-0119
local.identifier.orcidhttps://orcid.org/0000-0002-3680-6327
local.identifier.orcidhttps://orcid.org/0000-0002-4253-6992
local.identifier.orcidhttps://orcid.org/0000-0003-2721-3826
local.identifier.orcidhttps://orcid.org/0000-0001-8631-522X
local.identifier.orcidhttps://orcid.org/0000-0003-1738-1332
local.identifier.orcidhttps://orcid.org/0000-0002-8948-1722
local.identifier.orcidhttps://orcid.org/0000-0002-9153-1485
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.mdpi.com/1999-4915/12/2/207

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