Integrated proteomics, phosphoproteomics and metabolomics analyses reveal similarities among giant cell granulomas of the jaws with different genetic mutations

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dc.creatorJéssica Gardone Vitório
dc.creatorLiséte Celina Lange
dc.creatorLucilaine Valéria de Souza Santos
dc.creatorMartin Røssel Larsen
dc.creatorCarolina Cavalieri Gomes
dc.creatorRicardo Santiago Gomez
dc.creatorFilipe Fideles Duarte-Andrade
dc.creatorThais dos Santos Fontes Pereira
dc.creatorMarcella Nunes de Melo Braga
dc.creatorGisele André Baptista Canuto
dc.creatorAdriana Nori de Macedo
dc.creatorYuri Abner Rocha Lebron
dc.creatorVictor Rezende Moreira
dc.creatorLiza Figueiredo Felicori
dc.date.accessioned2023-10-27T22:06:54Z
dc.date.accessioned2025-09-09T00:41:29Z
dc.date.available2023-10-27T22:06:54Z
dc.date.issued2022
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipOutra Agência
dc.identifier.doihttps://doi.org/10.1111/jop.13327
dc.identifier.issn0904-2512
dc.identifier.urihttps://hdl.handle.net/1843/60209
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Oral Pathology & Medicine
dc.rightsAcesso Restrito
dc.subjectBiologia molecular
dc.subjectCélulas - Patogênese
dc.subjectTumores
dc.subjectPreparação de amostra (Química)
dc.subjectGranuloma
dc.subject.otherLesão central de células gigantes
dc.subject.otherMetabolômica
dc.subject.otherProteoma
dc.subject.otherBiologia molecular
dc.subject.otherBiologia de tumores
dc.titleIntegrated proteomics, phosphoproteomics and metabolomics analyses reveal similarities among giant cell granulomas of the jaws with different genetic mutations
dc.typeArtigo de periódico
local.citation.epage673
local.citation.issue7
local.citation.spage666
local.citation.volume51
local.description.resumoBackground: Giant cell granuloma of the jaws are benign osteolytic lesions of the jaws. These lesions are genetically characterized by mutually exclusive somatic mutations at TRPV4, KRAS, and FGFR1, and a fourth molecular subgroup which is wild-type for the three mutations. Irrespective of the molecular background, giant cell granulomas show MAPK/ERK activation. However, it remains unclear if these mutations lead to differences in their molecular signaling in giant cell granulomas. Methods: Metabolomics, proteomics, and phosphoproteomics analyses were carried out in formalin-fixed paraffin-embedded samples of giant cell granuloma of the jaws. The study cohort consisted of five lesions harboring mutations in FGFR1, six in KRAS, five in TRPV4, and five that were wild-type for these mutations. Results: Lesions harboring KRAS or FGFR1 mutations showed overall similar proteomics and metabolomics profiles. In all four groups, metabolic pathways showed similarity in apoptosis, cell signaling, gene expression, cell differentiation, and erythrocyte activity. Lesions harboring TRPV4 mutations showed a greater number of enriched pathways related to tissue architecture. On the other hand, the wild-type group presented increased number of enriched pathways related to protein metabolism compared to the other groups. Conclusion: Despite some minor differences, our results revealed an overall similar molecular profile among the groups with different mutational profile at the metabolic, proteic, and phosphopeptidic levels.
local.identifier.orcidhttps://orcid.org/0000-0001-6203-0123
local.identifier.orcidhttps://orcid.org/0000-0002-4125-3755
local.identifier.orcidhttps://orcid.org/0000-0003-0345-0376
local.identifier.orcidhttps://orcid.org/0000-0002-6174-8157
local.identifier.orcidhttps://orcid.org/0000-0002-5009-6675
local.identifier.orcidhttps://orcid.org/0009-0004-4352-1460
local.identifier.orcidhttps://orcid.org/0000-0001-6933-8454
local.identifier.orcidhttps://orcid.org/0000-0002-6616-3705
local.identifier.orcidhttps://orcid.org/0000-0002-3240-9658
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE PATOLOGIA
local.publisher.departmentICX - DEPARTAMENTO DE QUÍMICA
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/jop.13327

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