Comparative study on corneal epithelium healing: effects of crosslinked hyaluronic acid and amniotic membrane extract eye drops in rats

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dc.creatorLenara Gonçalves e Souza
dc.creatorMatheus Vilardo Loes Moreira
dc.creatorClaudia Sayuri Saçaki
dc.creatorEduardo Perlmann
dc.creatorThacyana Beatriz Guimarães Lopes
dc.creatorEnio Ferreira
dc.creatorJuan Carlos Duque Moreno
dc.creatorFabiano Montiani-Ferreira
dc.date.accessioned2026-01-06T20:57:22Z
dc.date.issued2024-07-23
dc.identifier.doihttps://doi.org/10.3389/fvets.2024.1415658
dc.identifier.issn2297-1769
dc.identifier.urihttps://hdl.handle.net/1843/1293
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofFrontiers in veterinary science
dc.rightsAcesso aberto
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectEstudo Comparativo
dc.subjectRatos
dc.subjectCicatrização
dc.subjectSoluções Oftálmicas
dc.subject.otherCross-linked hyaluronic acid hydrogel
dc.subject.otherAmniotic membrane extract
dc.subject.otherEye drops
dc.subject.otherEpithelial healing
dc.subject.otherRat
dc.subject.otherHistopathology
dc.titleComparative study on corneal epithelium healing: effects of crosslinked hyaluronic acid and amniotic membrane extract eye drops in rats
dc.typeArtigo de periódico
local.citation.epage11
local.citation.spage1
local.citation.volume11
local.description.resumoIntroduction: Corneal ulcers are common lesions in both human and veterinary medicine. However, only a few studies have evaluated the efficacy of cross-linked hyaluronic acid (X-HA) eye drops on corneal wound healing. To our knowledge, this is the first study to demonstrate and compare the efficacy of amniotic membrane extract eye drops (AMEED) and X-HA for corneal wound healing in rats.Material and methods: A total of 15 male Wistar rats (30 eyes) were used in this study. Then, 10 eyes were treated with X-HA, AMEED, or 0.9% saline. After general and topical anesthesia, a superficial corneal ulcer was created using a corneal trephine. The defect was further polished with a diamond burr. Three groups of 10 eyes each were treated with either one drop of 0.75% X-HA or AMEED or 0.9% saline (control), administered every 12 h for a duration of 72 h. The median epithelial defect area (MEDA), expressed as a percentage of the total corneal surface, was measured at 0, 12, 24, 36, 48, and 72 h. Re-epithelization time scores were also evaluated. The Kruskal–Wallis test was used to compare median times for re-epithelization and histopathologic scores between groups, while the Friedman test (for paired data) was employed to compare results from the serial analysis of MEDA and vascularization scores between groups.Results: MEDA was not significantly different between X-HA and AMEED. However, MEDA was significantly smaller in the X-HA group compared to the control group at 36 h (2.73 interquartile range (IQR) 5.52% x 9.95 IQR 9.10%, P=0.024) and 48 h (0.00 IQR 0.26% x 6.30 IQR 8.54%, P=0.030). The overall time for re-epithelization was significantly lower in the X-HA group (3.00 IQR 3.00) compared to the AMEED (6.5 IQR 3.00) and control (7.00 IQR 1.00) groups (P=0.035). Vascularization, hydropic degeneration, and epithelial-stromal separation were significantly less observed in samples in the X-HA-treated compared to samples in the AMEED- and saline-treated groups. Significantly more corneal epithelium cells were labeled for caspase3 in samples from the AMEED- and saline-treated groups compared to those from the X-HA-treated group.Discussion: Topical X-HA has been shown to accelerate corneal epithelial healing. AMEED did not decrease corneal re-epithelialization time. X-HA may also potentially be used as an adjunct therapy for treating corneal ulcers in clinical situations.
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE PATOLOGIA
local.publisher.initialsUFMG
local.subject.cnpqCIENCIAS AGRARIAS::MEDICINA VETERINARIA::PATOLOGIA ANIMAL
local.url.externahttps://www.frontiersin.org/journals/veterinary-science/articles/10.3389/fvets.2024.1415658/full

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