Selection and molecular identification of lactic acid bacteria with probiotic potential from girolando calves raised in a semiarid region
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Universidade Federal de Minas Gerais
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Diarrhea is one of the most important diseases in calves, resulting in high mortality along with the high costs of antimicrobial agents. For alternative control with probiotics, it is essential to isolate autochthonous bacteria that are effective against Escherichia coli. The aim of this study was to select lactic acid bacteria (LAB) showing probiotic potential from dairy calves raised in a semiarid tropical region. The feces of ten healthy weaned Girolando calves were sampled, and after dilution were incubated on solid de Man–Rogosa–Sharpe medium, in a microaerophilic atmosphere. A high population of LAB was observed in samples from each calf evaluated (average 2.8 × 109 colony-forming units g−. Of the 31 LAB isolates, all of which were negative for catalase production and and 61.3% were Gram-positive rods were Gram-positive rods, compatible with the Lactobacillus genus. However, only seven of these isolates showed milk coagulation capacity after successive peak and were pre-selected. In the test for screening resistance to bile salts at 1% and to pH4, growth was observed in three (42.8%) of the seven LAB. After 16S rRNA gene sequencing and proteomic analysis, a selected LAB identified as Lactobacillus plantarum enabled higher inhibition of E. coli of bovine origin than other isolates, presenting probiotic potential for the control of colibacillosis.
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Girolando (Bovino), Escherichia coli, Colibacilose, Diarreia em bovinos, Diarreia em bezerro
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https://www.fspublishers.org/html_issue.php?i_id=41675