Intratumor molecular heterogeneity in pleomorphic adenoma of the salivary glands

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dc.creatorCarolina Cavalieri Gomes
dc.creatorClarice Ferreira Galvão
dc.creatorAna Carolina de Melo do Carmo
dc.creatorNubia Braga Pereira
dc.creatorRicardo Santiago Gomez
dc.date.accessioned2025-06-05T19:59:11Z
dc.date.accessioned2025-09-09T00:33:46Z
dc.date.available2025-06-05T19:59:11Z
dc.date.issued2016-02
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1016/j.oooo.2015.09.007
dc.identifier.issn2212-4411
dc.identifier.urihttps://hdl.handle.net/1843/82833
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofOral Surgery, Oral Medicine, Oral Pathology and Oral Radiology
dc.rightsAcesso Restrito
dc.subjectCell proliferation
dc.subjectApoptosis
dc.subjectAssociation
dc.subjectEvaluation study
dc.subjectLoss of heterozygosity
dc.titleIntratumor molecular heterogeneity in pleomorphic adenoma of the salivary glands
dc.typeArtigo de periódico
local.citation.epage163
local.citation.issue2
local.citation.spage158
local.citation.volume121
local.description.resumoObjective: To assess intratumor molecular heterogeneity in salivary gland pleomorphic adenoma (PA) and to investigate if intratumor molecular heterogeneity is associated with cell proliferation or apoptotic indexes. Study design: Nine formalin-fixed paraffin-embedded samples of PA of salivary glands were included in the study. Cell proliferation was estimated by using Ki-67 immunohistochemistry, and apoptotic index was achieved by combining terminal deoxynucleotidyl transferase dUTP nick end-labeling with morphology. A minimum of two different tumor areas of each sample was microdissected, and DNA was extracted. DNA extracted from the tumor capsule was used as normal matched control. Different tumor areas were at least 4 mm from one another and comprised tumor cell-rich areas. Loss of heterozygosity was assessed by a panel of six polymorphic microsatellite markers located at chromosomes 3 (D3 S1029), 9 (D9 S162 and D9 S157), 11 (D11 S1369), and 17 (P53 and AFM238 WF2). Results: Six of nine samples showed intratumor heterogeneity on the basis of the loss of heterozygosity findings. Intratumor molecular heterogeneity did not show association with cell proliferation or apoptotic indexes (P > .05). Conclusions: Our findings point to the existence of intratumor molecular heterogeneity in salivary gland PA. This is an advance in the efforts to clarify PA molecular pathogenesis, showing that this characteristic is not exclusive to malignant solid tumors.
local.identifier.orcidhttps://orcid.org/0000-0003-1580-4995
local.identifier.orcidhttps://orcid.org/0000-0002-6714-3124
local.identifier.orcidhttps://orcid.org/0000-0001-8770-8009
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE PATOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S221244031501192X?via%3Dihub

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