Accelerated solvent extraction method for the quantification of polycyclic aromatic hydrocarbons in cocoa beans by gas chromatography–mass spectrometry

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dc.creatorRenata França Cassimiro Belo
dc.creatorJúlia Pereira Figueiredo
dc.creatorCarolina Mariana Nunes
dc.creatorRafael Pissinatti
dc.creatorScheilla Vitorino Carvalho de Souza
dc.creatorRoberto Gonçalves Junqueira
dc.date.accessioned2022-05-10T21:34:13Z
dc.date.accessioned2025-09-09T00:02:59Z
dc.date.available2022-05-10T21:34:13Z
dc.date.issued2017-05-15
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipOutra Agência
dc.identifier.doi10.1016/j.jchromb.2017.03.017
dc.identifier.issn1570-0232
dc.identifier.urihttps://hdl.handle.net/1843/41526
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Chromatography B
dc.rightsAcesso Restrito
dc.subjectTecnologia de alimentos
dc.subjectCacau
dc.subjectCromatografia gasosa
dc.subjectEspectrometria de massas
dc.subject.otherAccelerated solvent extraction
dc.subject.otherGas chromatography
dc.subject.otherMass spectrometry
dc.subject.otherPolycyclic aromatic hydrocarbons
dc.subject.otherSingle laboratory method validation
dc.subject.otherCocoa bean
dc.titleAccelerated solvent extraction method for the quantification of polycyclic aromatic hydrocarbons in cocoa beans by gas chromatography–mass spectrometry
dc.typeArtigo de periódico
local.citation.epage100
local.citation.spage87
local.citation.volume1053
local.description.resumoAn accelerated solvent extraction (ASE) procedure for use with gas chromatography-mass spectrometry (GC–MS) was optimized for the determination of eight polycyclic aromatic hydrocarbons (PAHs) in cocoa beans. Plackett-Burman and rotatable central composite design (RCCD) indicated that three variables affected the recoveries of PAHs during the extraction and purification steps: agitation time in the second liquid–liquid partition, weight of silica gel in the column, and volume of hexane for PAH elution from the column. After obtaining the optimal conditions, a single laboratory method validation was performed. Linearity was demonstrated for benzo[a]pyrene in the concentration range from 0.5 to 8.0 mg kg−1 of sample, corresponding to 1.25–20.0 μg kg−1 of cocoa on a fat basis. For the other analytes, linearity was observed from 0.75 to 8.0 μg kg−1 of sample (1.88–20.0 μg kg−1 of cocoa on a fat basis). Significant matrix effects were found for chrysene and benzo[b]fluoranthene. The precision of the method was verified with relative standard deviations (RSDs) ranging from 2.57 to 14.13% and from 4.36 to 19.77% under repeatability and intermediate precision conditions, respectively. The average recoveries of the eight PAHs ranged from 74.99 to 109.73%. These parameters, limits and measurement uncertainties met the performance criteria established by European Union regulations, except for the theoretical limit of detection for chrysene. The method was applied to the analysis of samples of Brazilian cocoa beans, and only one sample was found to have a PAH content above the maximum limit defined by the European Union legislation. This optimized and validated method is intended to be used as part of the official Brazilian monitoring programs investigating contaminants and residues in food.
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ALIMENTOS
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S1570023216310431?via%3Dihub

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