Optical and theoretical study of strand recognition by nucleic acid probes

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dc.creatorIvana Domljanovic
dc.creatorMaria Taskova
dc.creatorPâmella Miranda de Moura
dc.creatorGerald Weber
dc.creatorKira Astakhova
dc.date.accessioned2024-01-02T12:47:19Z
dc.date.accessioned2025-09-08T23:54:42Z
dc.date.available2024-01-02T12:47:19Z
dc.date.issued2020
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1038/s42004-020-00362-5
dc.identifier.issn2399-3669
dc.identifier.urihttps://hdl.handle.net/1843/62264
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofcommunications chemistry
dc.rightsAcesso Aberto
dc.subjectBiofísica
dc.subjectÁcidos nucleicos
dc.subjectÓtica
dc.subject.otherOptical and theoretical study
dc.subject.otherNucleic acid probes
dc.titleOptical and theoretical study of strand recognition by nucleic acid probes
dc.typeArtigo de periódico
local.citation.epage13
local.citation.issue1
local.citation.spage1
local.citation.volume3
local.description.resumoDetection of nucleic acids is crucial to the study of their basic properties and consequently to applying this knowledge to the determination of pathologies such as cancer. In this work, our goal is to determine new trends for creating diagnostic tools for cancer driver mutations. Herein, we study a library of natural and modified oligonucleotide duplexes by a combination of optical and theoretical methods. We report a profound effect of additives on the duplexes, including nucleic acids as an active crowder. Unpredictably and inconsistent with DNA+LNA/RNA duplexes, locked nucleic acids contribute poorly to mismatch discrimination in the DNA+LNA/DNA duplexes. We develop a theoretical framework that explains poor mismatch discrimination in KRAS oncogene. We implement our findings in a bead-bait genotyping assay to detect mutated human cancer RNA. The performance of rationally designed probes in this assay is superior to the LNA-primer polymerase chain reaction, and it agrees with sequencing data.
local.identifier.orcidhttps://orcid.org/0000-0002-9727-2496
local.identifier.orcidhttps://orcid.org/0000-0001-8876-1864
local.identifier.orcidhttps://orcid.org/0000-0002-2935-1571
local.identifier.orcidhttps://orcid.org/0000-0003-4878-0301
local.publisher.countryBrasil
local.publisher.departmentICX - DEPARTAMENTO DE FÍSICA
local.publisher.initialsUFMG
local.url.externahttps://www.nature.com/articles/s42004-020-00362-5

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