Guidelines for the purification and characterization of extracellularvesicles of parasites

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dc.creatorCarmen Fernandez Becerra
dc.creatorNuria Cortes Serra
dc.creatorPaula Abou Karam
dc.creatorPaula Meneghetti
dc.creatorRafael Pedro Madeira
dc.creatorZiv Porat
dc.creatorRodrigo Soares
dc.creatorAdriana Oliveira Costa
dc.creatorSima Rafati
dc.creatorAnabela Cordeiro-da-Silva
dc.creatorNuno Santarém
dc.creatorPatricia Xander
dc.creatorChristopher Fernandez-Prada
dc.creatorDaniel Alfandari
dc.creatorGeorge Dong
dc.creatorIris Aparici
dc.creatorIrit Rosenhek-Goldian
dc.creatorMehrdad Shokouhy
dc.creatorMelisa Gualdron-lopez
dc.creatorNicholy Lozano
dc.creatorLysangela Alves
dc.creatorHernando del Portillo Obando
dc.creatorMelisa Gualdrón-López
dc.creatorAna Claudia Torrecilhas
dc.creatorSergio Schenkman
dc.creatorMartin Olivier
dc.creatorIgor Almeida
dc.creatorMarcel Ramirez
dc.creatorDolores Bernal
dc.creatorAntonio Marcilla
dc.creatorNeta Regev-Rudzki
dc.date.accessioned2025-01-24T22:01:19Z
dc.date.accessioned2025-09-08T22:50:37Z
dc.date.available2025-01-24T22:01:19Z
dc.date.issued2023-10-19
dc.format.mimetypepdf
dc.identifier.doihttps://doi.10.1002/jex2.117
dc.identifier.issn2768-2811
dc.identifier.urihttps://hdl.handle.net/1843/79486
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Extracellular Biology
dc.rightsAcesso Aberto
dc.subjectDoenças parasitárias
dc.subjectHelminto
dc.subjectProtozoário patogênico
dc.subjectInfecção
dc.subject.otherEVs methodology
dc.subject.otherExtracellular vesicles
dc.subject.otherHost-parasite interaction
dc.subject.otherInfection
dc.subject.otherProtozoan parasites
dc.subject.otherHelminth
dc.titleGuidelines for the purification and characterization of extracellularvesicles of parasites
dc.typeArtigo de periódico
local.citation.epage74
local.citation.issue10
local.citation.spage1
local.citation.volume2
local.description.resumoParasites are responsible for the most neglected tropical diseases, affecting over a bil-lion people worldwide (WHO, 2015) and accounting for billions of cases a year andresponsible for several millions of deaths. Research on extracellular vesicles (EVs)has increased in recent years and demonstrated that EVs shed by pathogenic para-sites interact with host cells playing an important role in the parasite’s survival, suchas facilitation of infection, immunomodulation, parasite adaptation to the host envi-ronment and the transfer of drug resistance factors. Thus, EVs released by parasitesmediate parasite-parasite and parasite-host intercellular communication. In addi-tion, they are being explored as biomarkers of asymptomatic infections and diseaseprognosis after drug treatment. However, most current protocols used for the iso-lation, size determination, quantification and characterization of molecular cargo ofEVs lack greater rigor, standardization, and adequate quality controls to certify theenrichment or purity of the ensuing bioproducts. We are now initiating major guide-lines based on the evolution of collective knowledge in recent years. The main pointscovered in this position paper are methods for the isolation and molecular character-ization of EVs obtained from parasite-infected cell cultures, experimental animals,and patients. The guideline also includes a discussion of suggested protocols andfunctional assays in host cells
local.identifier.orcidhttps://orcid.org/0000-0001-5154-0013
local.identifier.orcidhttps://orcid.org/0000-0001-5396-3757
local.identifier.orcidhttps://orcid.org/0000-0001-6612-4936
local.identifier.orcidhttps://orcid.org/0000-0001-9885-3818
local.identifier.orcidhttps://orcid.org/0000-0001-7319-2929
local.identifier.orcidhttps://orcid.org/0000-0003-3059-181X
local.identifier.orcidhttps://orcid.org/0000-0002-7966-3629
local.identifier.orcidhttps://orcid.org/0000-0002-1793-3013
local.identifier.orcidhttps://orcid.org/0000-0002-7221-1320
local.identifier.orcidhttps://orcid.org/0000-0003-2392-6087
local.identifier.orcidhttps://orcid.org/0000-0002-6329-6449
local.identifier.orcidhttps://orcid.org/0000-0003-4834-4956
local.identifier.orcidhttps://orcid.org/0000-0002-4878-5359
local.identifier.orcidhttps://orcid.org/0000-0003-0583-0698
local.identifier.orcidhttps://orcid.org/0000-0002-8202-6751
local.identifier.orcidhttps://orcid.org/0000-0001-5724-2199
local.identifier.orcidhttps://orcid.org/0000-0001-9353-8480
local.identifier.orcidhttps://orcid.org/0000-0002-3901-6638
local.identifier.orcidhttps://orcid.org/0000-0002-6917-1954
local.identifier.orcidhttps://orcid.org/0000-0003-4627-8393
local.identifier.orcidhttps://orcid.org/0000-0003-0004-0531
local.identifier.orcidhttps://orcid.org/0000-0001-7252-0193
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ANÁLISES CLÍNICAS E TOXICOLÓGICAS
local.publisher.initialsUFMG
local.url.externahttps://isevjournals.onlinelibrary.wiley.com/doi/full/10.1002/jex2.117

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