Steric mass action model for lactoferrin adsorption in cryogel with immobilized copper ions

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dc.creatorBruna Mara Aparecida de Carvalho
dc.creatorWiller Ferreira Silva Júnior
dc.creatorLorendane Millena Carvalho
dc.creatorLuis Antonio Minim
dc.creatorGleidson Giordano Pinto de Carvalho
dc.date.accessioned2022-06-23T15:24:14Z
dc.date.accessioned2025-09-09T01:16:03Z
dc.date.available2022-06-23T15:24:14Z
dc.date.issued2016
dc.identifier.doihttps://doi.org/10.1590/0104-6632.20160331s20140112
dc.identifier.issn0104-6632
dc.identifier.urihttps://hdl.handle.net/1843/42620
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofBrazilian Journal of Chemical Engineering
dc.rightsAcesso Aberto
dc.subjectCromatografia de afinidade
dc.subjectProteínas
dc.subjectGlicoproteinas
dc.titleSteric mass action model for lactoferrin adsorption in cryogel with immobilized copper ions
dc.typeArtigo de periódico
local.citation.epage223
local.citation.issue1
local.citation.spage215
local.citation.volume33
local.description.resumoParameters of equilibrium adsorption obtained from experiments using immobilized metal affinity chromatography (IMAC) were used to evaluate the applicability of the steric mass-action (SMA) model to describe the adsorption of lactoferrin to cryogel resin under different conditions. The adsorption of lactoferrin on continuous supermacroporous cryogel with immobilized Cu2+ ions was evaluated in batch adsorption experiments at different pH (6-8) and temperature (293-313 K) values. Estimated values of the equilibrium constant (K) and characteristic number of binding sites (n) showed that these parameters decreased with increasing ionic strength, pH and temperature, while the nonlinear parameter, the steric factor (σ), increased with increasing ionic strength and temperature. Expressions correlating these parameters with pH, ionic strength and temperature were then derived.
local.identifier.orcidhttps://orcid.org/0000-0001-8980-8599
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://www.scielo.br/j/bjce/a/VvnCb5bpsSsgPSvrxmnpYgh/?lang=en

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