Gestational age‐dependent gene expression profiling of ATP‐binding cassette transporters in the healthy human placenta

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dc.creatorGüínever Eustáquio do Império
dc.creatorTania Maria Ortiga Carvalho
dc.creatorEnrrico Bloise
dc.creatorMohsen Javam
dc.creatorPhetcharawan Lye
dc.creatorAndrea Constantinof
dc.creatorCaroline E. Dunk
dc.creatorFernando Marcos Dos Reis
dc.creatorStephen Lye
dc.creatorWilliam Gibb
dc.creatorStephen Matthews
dc.date.accessioned2023-10-05T23:04:41Z
dc.date.accessioned2025-09-09T00:43:10Z
dc.date.available2023-10-05T23:04:41Z
dc.date.issued2019
dc.identifier.doihttps://doi.org/10.1111/jcmm.13966
dc.identifier.issn1582-1838
dc.identifier.urihttps://hdl.handle.net/1843/59243
dc.languagepor
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Cellular and Molecular Medicine
dc.rightsAcesso Aberto
dc.subjectPlacenta
dc.subjectTransportadores de Cassetes de Ligação de ATP
dc.subject.otherABC transporters
dc.subject.otherABCA6
dc.subject.otherGestational-age
dc.subject.otherLipid transporter
dc.subject.otherPlacenta
dc.titleGestational age‐dependent gene expression profiling of ATP‐binding cassette transporters in the healthy human placenta
dc.typeArtigo de periódico
local.citation.epage618
local.citation.issue1
local.citation.spage610
local.citation.volume23
local.description.resumoThe ATP-binding cassette (ABC) transporters control placental transfer of several nutrients, steroids, immunological factors, chemicals, and drugs at the maternal-fetal interface. We and others have demonstrated a gestational age-dependent expression pattern of two ABC transporters, P-glycoprotein and breast cancer resistance protein throughout pregnancy. However, no reports have comprehensively elucidated the expression pattern of all 50 ABC proteins, comparing first trimester and term human placentae. We hypothesized that placental ABC transporters are expressed in a gestational-age dependent manner in normal human pregnancy. Using the TaqMan® Human ABC Transporter Array, we assessed the mRNA expression of all 50 ABC transporters in first (first trimester, n = 8) and third trimester (term, n = 12) human placentae and validated the resulting expression of selected ABC transporters using qPCR, Western blot and immunohistochemistry. A distinct gene expression profile of 30 ABC transporters was observed comparing first trimester vs. term placentae. Using individual qPCR in selected genes, we validated the increased expression of ABCA1 (P < 0.01), ABCA6 (P < 0.001), ABCA9 (P < 0.001) and ABCC3 (P < 0.001), as well as the decreased expression of ABCB11 (P < 0.001) and ABCG4 (P < 0.01) with advancing gestation. One important lipid transporter, ABCA6, was selected to correlate protein abundance and characterize tissue localization. ABCA6 exhibited increased protein expression towards term and was predominantly localized to syncytiotrophoblast cells. In conclusion, expression patterns of placental ABC transporters change as a function of gestational age. These changes are likely fundamental to a healthy pregnancy given the critical role that these transporters play in the regulation of steroidogenesis, immunological responses, and placental barrier function and integrity.
local.identifier.orcidhttps://orcid.org/0000-0002-5744-6623
local.identifier.orcidhttps://orcid.org/0000-0003-0275-8686
local.identifier.orcidhttps://orcid.org/0000-0002-4146-099X
local.identifier.orcidhttps://orcid.org/0000-0002-3305-4406
local.identifier.orcidhttps://orcid.org/0000-0001-5063-1773
local.publisher.countryBrasil
local.publisher.departmentMED - DEPARTAMENTO DE GINECOLOGIA OBSTETRÍCIA
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/full/10.1111/jcmm.13966

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