Establishing tools for early diagnosis of congenital toxoplasmosis: flow cytometric igg avidity assay as a confirmatory test for neonatal screening

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dc.creatorAlinede Castro Zacche-tonini
dc.creatorDaniel Vítor de Vasconcelos Santos
dc.creatorJosé Nélio Januário
dc.creatorAndréa Teixeira-carvalho
dc.creatorRicardo Wagner Almeida Vitor
dc.creatorEloísa Amália Vieira Ferro
dc.creatorJosé Roberto Mineo
dc.creatorOlindo Assis Martins-filho
dc.creatorElenice Moreira Lemos
dc.creatorGiuliana Schmidt França Fonseca
dc.creatorLaura Néspoli Nassar Passini de Jesus
dc.creatorGeisa Baptista Barros
dc.creatorJordana Grazziela Alves Coelho-dos-reis
dc.creatorSamantha Ribeiro Béla
dc.creatorAnderson Silva Machado
dc.creatorAna Carolina Aguiar Vasconcelos Carneiro
dc.creatorGláucia Manzan Queiroz Andrade
dc.date.accessioned2023-06-23T22:22:32Z
dc.date.accessioned2025-09-09T01:04:21Z
dc.date.available2023-06-23T22:22:32Z
dc.date.issued2017-08-18
dc.format.mimetypepdf
dc.identifier.doi10.1016/j.jim.2017.08.005
dc.identifier.issn00221759
dc.identifier.urihttps://hdl.handle.net/1843/55299
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Immunological Methods
dc.rightsAcesso Restrito
dc.subjectCitometria de Fluxo
dc.subjectImunoglobulina G
dc.subject.otherCongenital T. gondii infection
dc.subject.otherFlow cytometry
dc.subject.otherSerological diagnosis
dc.subject.otherIgG
dc.titleEstablishing tools for early diagnosis of congenital toxoplasmosis: flow cytometric igg avidity assay as a confirmatory test for neonatal screening
dc.typeArtigo de periódico
local.citation.epage47
local.citation.issue2017
local.citation.spage37
local.citation.volume451
local.description.resumoThe aim of this study was to evaluate the performance of conventional serology (Q-Preven™ and ELFAVIDAS™)and flow cytometry-based serologic tools for early serologic diagnosis of congenital toxoplasmosis. The study groups included prospectively confirmed cases of congenital toxoplasmosis (TOXO = 88) and age-matching non infected controls (NI = 15).The results demonstrated that all samples tested positive/indeterminate for anti-T.gondii IgM screening at birth using air-dried whole blood samples. Serum samples collected at 30–45 days after birth tested positive for ELFAVIDAS™ IgG in both groups. While all NI tested negative for ELFAVIDAS™ IgM and IgA, only 78% and 36% of TOXO tested positive for IgM and IgA, respectively. Flow cytometry-based anti-T. gondii IgM, IgA and IgG reactivity displayed moderate performance with low sensitivity (47.6%, 72.6% and 75.0%, respectively). Regardless the remarkable specificity of IgG1, IgG2 and IgG3 subclasses for early diagnosis,weak or moderate specificity was observed (Se = 73.9%, 60.2% and 83.0%, respectively). The analysis of IgG avidity indices (AI) demonstrated the highest performance among the flow cytometry-based methods (Se = 96.6%; Sp = 93.3%), underscoring the low avidity index (AI < 60%) within TOXO (97.0%) in contrast with the high avidity index (AI > 60%) in NI (93%). Analysis of anti-T. gondii IgG and IgG3 reactivity for mother:infant paired samples may represent a relevant complementary tests for early diagnosis. In conclusion, afeasible high-standard algorithm (Accuracy = 97.1%) was proposed consisting of Q-Preven™ IgM screening at birth, followed by ELFAVIDAS™ IgM and flow cytometric IgG avidity analysis at 30–45 days after birth as a highperformance tool for early serological diagnosis of congenital toxoplasmosis.
local.identifier.orcidhttps://orcid.org/0000-0002-0912-3782
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE PARASITOLOGIA
local.publisher.departmentMED - DEPARTAMENTO DE CLÍNICA MÉDICA
local.publisher.departmentMED - DEPARTAMENTO DE OFTALMOLOGIA E OTORRINOLARINGOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S002217591730282X?via%3Dihub

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