Genotyping analysis of cryptococcus deuterogattii and correlation with virulence factors and antifungal susceptibility by the clinical and laboratory standards Institute and the European Committee on antifungal susceptibility testing methods

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dc.creatorLeonardo Euripedes Andrade Silva
dc.creatorAnderson Vilas Boas
dc.creatorKennio Ferreira Paim
dc.creatorJuliana Andrade Silva
dc.creatorDaniel de Assis Santos
dc.creatorThatiana Bragine Ferreira
dc.creatorAercio Sebastião Borges
dc.creatorDelio Jose Mora
dc.creatorMarcia de Souza Carvalho Melhem
dc.creatorMario Léon Silva Vergara
dc.date.accessioned2025-01-21T21:32:40Z
dc.date.accessioned2025-09-08T23:05:37Z
dc.date.available2025-01-21T21:32:40Z
dc.date.issued2023-08-31
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.3390/jof9090889
dc.identifier.issn2309-608X
dc.identifier.urihttps://hdl.handle.net/1843/79387
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Fungi
dc.rightsAcesso Aberto
dc.subjectAtividade antifúngica
dc.subjectGenótipo
dc.subjectVirulencia (Microbiologia)
dc.subject.otherCryptococcus gattii species complex (CGSC)
dc.subject.otherCryptococcus deuterogattii
dc.subject.otherCLSI
dc.subject.otherEUCAST
dc.subject.otherVirulence factors
dc.titleGenotyping analysis of cryptococcus deuterogattii and correlation with virulence factors and antifungal susceptibility by the clinical and laboratory standards Institute and the European Committee on antifungal susceptibility testing methods
dc.typeArtigo de periódico
local.citation.epage17
local.citation.issue9
local.citation.spage1
local.citation.volume9
local.description.resumoData about the relationship between their molecular types, virulence factors, clinical presentation, antifungal susceptibility profile, and outcome are still limited for Cryptococcus deuterogattii. This study aimed to evaluate the molecular and phenotypic characteristics of 24 C. deuterogattii isolates from the southeast region of Brazil. The molecular characterization was performed by multilocus sequence typing (MLST). The antifungal susceptibility profile was obtained according to CLSI-M27-A3 and EUCAST-EDef 7.1 methods. The virulence factors were evaluated using classic techniques. The isolates were divided into four populations. The molecular analysis suggests recombinant events in most of the groups evaluated. Resistance and susceptibility dose-dependent to fluconazole were evidenced in four isolates (16%) by EUCAST and in four isolates (16%) by CLSI methods. The agreement at ±two dilutions for both methods was 100% for itraconazole, ketoconazole, and voriconazole, 96% for amphotericin B, and 92% for fluconazole. Significant differences in virulence factor expression and antifungal susceptibility to itraconazole and amphotericin B were found. The mixed infection could be suggested by the presence of variable sequence types, differences in virulence factor production, and decreased antifungal susceptibility in two isolates from the same patient. The data presented herein corroborate previous reports about the molecular diversity of C. deuterogattii around the world.
local.identifier.orcidhttps://orcid.org/0000-0002-0485-229X
local.identifier.orcidhttps://orcid.org/0000-0002-6035-8392
local.identifier.orcidhttps://orcid.org/0000-0002-7627-3000
local.identifier.orcidhttps://orcid.org/0000-0002-1108-5666
local.identifier.orcidhttps://orcid.org/0000-0002-4038-0744
local.identifier.orcidhttps://orcid.org/0000-0002-1335-8808
local.identifier.orcidhttps://orcid.org/0000-0003-4296-9963
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE MICROBIOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.mdpi.com/2309-608X/9/9/889

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