Identification and characterisation of SARS-CoV-2 and human alphaherpesvirus 1 from a productive coinfection in a fatal Covid-19 case

Página do item simplificado
dc.creatorAlice Laschuk Herlinger
dc.creatorCarolina Moreira Voloch
dc.creatorAdriana Suely de Oliveira Melo
dc.creatorRenato Santana de Aguiar
dc.creatorAndré Felipe Andrade dos Santos
dc.creatorTerezinha Marta Pereira Pinto Castiñeiras
dc.creatorAna Tereza Ribeiro de Vasconcelos
dc.creatorEsaú Custódio João Filho
dc.creatorClaudia Caminha Escosteguy
dc.creatorOrlando da Costa Ferreira Junior
dc.creatorAmilcar Tanuri
dc.creatorFábio Luís Lima Monteiro
dc.creatorLuiza Mendonça Higa
dc.creatorMirela D'arc
dc.creatorFilipe Romero Rebello Moreira
dc.creatorHarrison James Westgarth
dc.creatorRafael Mello Galliez
dc.creatorDiana Mariani
dc.creatorLuciana Jesus da Costa
dc.creatorLuiz Gonzaga Paula de Almeida
dc.date.accessioned2025-01-23T20:44:08Z
dc.date.accessioned2025-09-08T23:54:56Z
dc.date.available2025-01-23T20:44:08Z
dc.date.issued2022-01-10
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1590/0074-02760210176
dc.identifier.issn1678-8060
dc.identifier.urihttps://hdl.handle.net/1843/79440
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofMemórias do Instituto Oswaldo Cruz
dc.rightsAcesso Aberto
dc.subjectCOVID-19 (Doença)
dc.subjectCoinfecção
dc.subjectSARS-CoV-2
dc.subject.otherSARS-CoV-2
dc.subject.otherCOVID-19
dc.subject.otherHSV-1
dc.subject.otherCoinfection
dc.subject.otherCoronavirus
dc.titleIdentification and characterisation of SARS-CoV-2 and human alphaherpesvirus 1 from a productive coinfection in a fatal Covid-19 case
dc.typeArtigo de periódico
local.citation.epage9
local.citation.spage1
local.citation.volume116
local.description.resumoBACKGROUND During routine Coronavirus disease 2019 (COVID-19) diagnosis, an unusually high viral load was detected by reverse transcription real-time polymerase chain reaction (RT-qPCR) in a nasopharyngeal swab sample collected from a patient with respiratory and neurological symptoms who rapidly succumbed to the disease. Therefore we sought to characterise the infection. OBJECTIVES We aimed to determine and characterise the etiological agent responsible for the poor outcome. METHODS Classical virological methods, such as plaque assay and plaque reduction neutralisation test combined with ampliconbased sequencing, as well as a viral metagenomic approach, were performed to characterise the etiological agents of the infection. FINDINGS Plaque assay revealed two distinct plaque phenotypes, suggesting either the presence of two severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) strains or a productive coinfection of two different species of virus. Amplicon-based sequencing did not support the presence of any SARS-CoV-2 genetic variants that would explain the high viral load and suggested the presence of a single SARS-CoV-2 strain. Nonetheless, the viral metagenomic analysis revealed that Coronaviridae and Herpesviridae were the predominant virus families within the sample. This finding was confirmed by a plaque reduction neutralisation test and PCR. MAIN CONCLUSIONS We characterised a productive coinfection of SARS-CoV-2 and Herpes simplex virus 1 (HSV-1) in a patient with severe symptoms that succumbed to the disease. Although we cannot establish the causal relationship between the coinfection and the severity of the clinical case, this work serves as a warning for future studies focused on the interplay between SARS-CoV-2 and HSV-1 coinfection and COVID-19 severity.
local.publisher.countryBrasil
local.publisher.departmentICB - INSTITUTO DE CIÊNCIAS BIOLOGICAS
local.publisher.initialsUFMG
local.url.externahttps://www.scielo.br/j/mioc/a/MgHwBf6HDtZwTpC8PT5YCDP/#

Arquivos

Pacote original

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
Identification and characterisation of sars-cov-2 and human alphaherpesvirus 1 from a productive coinfection in a fatal covid-19 case.pdf
Tamanho:
1.34 MB
Formato:
Adobe Portable Document Format
Baixar

Licença do pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
License.txt
Tamanho:
1.99 KB
Formato:
Plain Text
Descrição:
Baixar

Coleções

Artigo de Periódico

Logo UFMG