Oral Tolerance Induced by Heat Shock Protein 65-Producing Lactococcus lactis Mitigates Inflammation in Leishmania braziliensis Infection

Página do item simplificado
dc.creatorPriscila Valera Guerra
dc.creatorTatiani Uceli Maiolii
dc.creatorAna Maria Caetano Faria
dc.creatorCláudia Ida Brodskyn
dc.creatorCamila Mattos Andrade
dc.creatorIvanéia Valeriano Nunes
dc.creatorBrena Cardoso Gama
dc.creatorRafael Tibúrcio
dc.creatorWashington Luis Conrado Santos
dc.creatorVasco Ariston de Carvalho Azevedo
dc.creatorNatalia Machado Tavares
dc.creatorJuliana de Souza Rebouças
dc.date.accessioned2024-03-21T14:19:18Z
dc.date.accessioned2025-09-09T01:14:15Z
dc.date.available2024-03-21T14:19:18Z
dc.date.issued2021-06-23
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipOutra Agência
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.3389/fimmu.2021.647987
dc.identifier.issn1664-3224
dc.identifier.urihttps://hdl.handle.net/1843/66317
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofFrontiers in Immunology
dc.rightsAcesso Aberto
dc.subjectLeishmania braziliensis
dc.subjectResposta ao Choque Térmico
dc.subjectLactococcus lactis
dc.subjectInterleucina-10
dc.subjectReceptor 2 Toll-Like
dc.subjectFator de Crescimento Transformador beta
dc.subject.otherLeishmania braziliensis
dc.subject.otherHeat shock protein 65
dc.subject.otherLactococcus lactis
dc.subject.otherOral tolerance
dc.subject.otherIL-10
dc.subject.otherTLR2
dc.subject.otherTGF-b
dc.titleOral Tolerance Induced by Heat Shock Protein 65-Producing Lactococcus lactis Mitigates Inflammation in Leishmania braziliensis Infection
dc.typeArtigo de periódico
local.citation.epage15
local.citation.spage1010010
local.citation.volume12
local.description.resumoCutaneous leishmaniasis caused by L. braziliensis induces a pronounced Th1 inflammatory response characterized by IFN-γ production. Even in the absence of parasites, lesions result from a severe inflammatory response in which inflammatory cytokines play an important role. Different approaches have been used to evaluate the therapeutic potential of orally administrated heat shock proteins (Hsp). These proteins are evolutionarily preserved from bacteria to humans, highly expressed under inflammatory conditions and described as immunodominant antigens. Tolerance induced by the oral administration of Hsp65 is capable of suppressing inflammation and inducing differentiation in regulatory cells, and has been successfully demonstrated in several experimental models of autoimmune and inflammatory diseases. We initially administered recombinant Lactococcus lactis (L. lactis) prior to infection as a proof of concept, in order to verify its immunomodulatory potential in the inflammatory response arising from L. braziliensis. Using this experimental approach, we demonstrated that the oral administration of a recombinant L. lactis strain, which produces and secretes Hsp65 from Mycobacterium leprae directly into the gut, mitigated the effects of inflammation caused by L. braziliensis infection in association or not with PAM 3CSK4 (N-α-Palmitoyl-S-[2,3-bis(palmitoyloxy)-(2RS)-propyl]-L-cysteine, a TLR2 agonist). This was evidenced by the production of anti-inflammatory cytokines and the expansion of regulatory T cells in the draining lymph nodes of BALB/c mice. Our in vitro experimental results suggest that IL-10, TLR-2 and LAP are important immunomodulators in L. braziliensis infection. In addition, recombinant L. lactis administered 4 weeks after infection was observed to decrease lesion size, as well as the number of parasites, and produced a higher IL-10 production and decrease IFN-γ secretion. Together, these results indicate that Hsp65-producing L. lactis can be considered as an alternative candidate for treatment in both autoimmune diseases, as well as in chronic infections that cause inflammatory disease.
local.identifier.orcidhttps://orcid.org/0000-0002-7538-3208
local.publisher.countryBrasil
local.publisher.departmentENF - DEPARTAMENTO DE NUTRIÇÃO
local.publisher.departmentENFERMAGEM - ESCOLA DE ENFERMAGEM
local.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.frontiersin.org/journals/immunology/articles/10.3389/fimmu.2021.647987/full

Arquivos

Pacote original

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
Oral Tolerance Induced by Heat Shock Protein 65-Producing Lactococcus lactis Mitigates Inflammation in Leishmania braziliensis Infection.pdf
Tamanho:
1.65 MB
Formato:
Adobe Portable Document Format
Baixar

Licença do pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
License.txt
Tamanho:
1.99 KB
Formato:
Plain Text
Descrição:
Baixar

Coleções

Artigo de Periódico

Logo UFMG