Leptin impairs the therapeutic effect of ionizing radiation inoral squamous cell carcinoma cells

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dc.creatorRogério Gonçalves da Rocha
dc.creatorEliane Macedo Sobrinho Santos
dc.creatorEloá Mangabeira Santos
dc.creatorEmisael Stênio Batista Gomes
dc.creatorGuilherme Veloso Ramos
dc.creatorKarina Marini Aguiar
dc.creatorBruno Rodrigues Gonçalves
dc.creatorSérgio Henrique Sousa Santos
dc.creatorAlfredo Maurício Batista de Paula
dc.creatorAndré Luiz Sena Guimarães
dc.creatorLucyana Conceição Farias
dc.date.accessioned2022-08-19T13:21:19Z
dc.date.accessioned2025-09-09T01:30:02Z
dc.date.available2022-08-19T13:21:19Z
dc.date.issued2019-01
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.identifier.doihttps://doi.org/10.1111/jop.12786
dc.identifier.issn1600-0714
dc.identifier.urihttps://hdl.handle.net/1843/44385
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Oral Pathology and Medicine
dc.rightsAcesso Restrito
dc.subjectCâncer
dc.subjectRadiação ionizante
dc.subjectHomeostase
dc.subjectCélulas - Proliferação
dc.subjectCarcinogênese
dc.titleLeptin impairs the therapeutic effect of ionizing radiation inoral squamous cell carcinoma cells
dc.typeArtigo de periódico
local.citation.epage23
local.citation.issue1
local.citation.spage17
local.citation.volume48
local.description.resumoPurpose: leptin, an important hormone controlling energy homeostasis, has been linked to the pathogenesis of oral squamous cell carcinoma (OSCC). Evidence indicates that head and neck cancer patients undergoing radiotherapy show decreased leptin levels after radiotherapy treatment. Thus, we investigated, through phenotypic and molecular analyses, whether leptin can compromise the therapeutic effect of ionizing radiation and neoplastic behavior of OSCC cells. Methods: the human OSCC-derived cell lines SCC9 and SCC4 were treated with human recombinant leptin and exposed to 6 Gy of irradiation. We performed the in vitro assays of cell migration, death, proliferation, and colony-forming ability. The reactive oxygen species (ROS) levels and proteome analysis by mass spectrometry were also conducted. Results: leptin was able to increase cell proliferation, migration, and colony-forming ability, despite the suppressive effect induced by irradiation. Furthermore, the leptin promoted a significant reduction of ROS intracellular accumulation, and increased expression of the cancer-related proteins, as ACTC1, KRT6A, and EEF2 in irradiated OSCC cells. Conclusions: our findings suggest that leptin impairs responsivity of OSCC cells to the ionizing radiation, reducing the suppressive effects of irradiation on the neoplastic phenotype, and increasing protein expression critical to carcinogenesis.
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/jop.12786

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