A simple, fast and sensitive screening LC-ESI-MS/MS method for antibiotics in fish

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dc.creatorLetícia Rocha Guidi
dc.creatorFlávio Alves Santos
dc.creatorAna Cláudia dos Santos Rossi Ribeiro
dc.creatorChristian Fernandes
dc.creatorLuiza Helena Meller da Silva
dc.creatorMaria Beatriz de Abreu Glória
dc.date.accessioned2022-03-28T21:05:36Z
dc.date.accessioned2025-09-09T01:02:09Z
dc.date.available2022-03-28T21:05:36Z
dc.date.issued2017-01-15
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doi10.1016/j.talanta.2016.10.089
dc.identifier.issn00399140
dc.identifier.urihttps://hdl.handle.net/1843/40516
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofTalanta (oxford)
dc.rightsAcesso Aberto
dc.subjectFarmácia
dc.subjectPeixes
dc.subjectAntibióticos
dc.subject.otherAminoglycoside
dc.subject.otherBeta-Lactam
dc.subject.otherMacrolide
dc.subject.otherQuinolone
dc.subject.otherSulfonamide
dc.subject.otherAquaculture
dc.titleA simple, fast and sensitive screening LC-ESI-MS/MS method for antibiotics in fish
dc.typeArtigo de periódico
local.citation.epage93
local.citation.spage85
local.citation.volume163
local.description.resumoThe objective of this study was to develop and validate a fast, sensitive and simple liquid chromatography–electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) method for the screening of six classes of antibiotics (aminoglycosides, beta-lactams, macrolides, quinolones, sulfonamides and tetracyclines) in fish. Samples were extracted with trichloroacetic acid. LC separation was achieved on a Zorbax Eclipse XDB C18 column and gradient elution using 0.1% heptafluorobutyric acid in water and acetonitrile as mobile phase. Analysis was carried out in multiple reaction monitoring mode via electrospray interface operated in the positive ionization mode, with sulfaphenazole as internal standard. The method was suitable for routine screening purposes of 40 antibiotics, according to EC Guidelines for the Validation of Screening Methods for Residues of Veterinary Medicines, taking into consideration threshold value, cut-off factor, detection capability, limit of detection, sensitivity and specificity. Real fish samples (n=193) from aquaculture were analyzed and 15% were positive for enrofloxacin (quinolone), one of them at a higher concentration than the level of interest (50 µg kg−1), suggesting possible contamination or illegal use of that antibiotic.
local.publisher.countryBrasil
local.publisher.departmentFAR - DEPARTAMENTO DE ALIMENTOS
local.publisher.departmentFAR - DEPARTAMENTO DE PRODUTOS FARMACÊUTICOS
local.publisher.initialsUFMG
local.url.externahttps://www.sciencedirect.com/science/article/pii/S0039914016308451

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