BRAF p.V600E status in epithelial areas of ameloblastoma with different histological aspects: implications to the clinical practice

Página do item simplificado
dc.creatorMaria Sissa Pereira Sant'ana
dc.creatorSara Ferreira dos Santos Costa
dc.creatorMaísa Pereira da Silva
dc.creatorRoberta Rayra Martins Chaves
dc.creatorThaís dos Santos Fontes Pereira
dc.creatorEduardo Morato de Oliveira
dc.creatorRicardo Martínez Pedraza
dc.creatorWagner Henriques de Castro
dc.creatorCarolina Cavaliéri Gomes
dc.creatorRicardo Santiago Gomez
dc.creatorFelipe Paiva Fonseca
dc.date.accessioned2025-01-31T14:01:21Z
dc.date.accessioned2025-09-08T23:13:19Z
dc.date.available2025-01-31T14:01:21Z
dc.date.issued2021-05
dc.identifier.doihttps://doi.org/10.1111/jop.13155
dc.identifier.issn1600-0714
dc.identifier.urihttps://hdl.handle.net/1843/79569
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Oral Pathology & Medicine
dc.rightsAcesso Restrito
dc.subjectAmeloblastoma
dc.subjectProto-oncogene proteins B-raf
dc.subjectDentigerous cyst
dc.subjectOdontogenic tumors
dc.subject.otherBRAF
dc.subject.otherBRAF p.V600E
dc.subject.otherAmeloblastoma
dc.subject.otherDentigerous cyst
dc.subject.otherOdontogenic tumour
dc.titleBRAF p.V600E status in epithelial areas of ameloblastoma with different histological aspects: implications to the clinical practice
dc.typeArtigo de periódico
local.citation.epage484
local.citation.issue5
local.citation.spage478
local.citation.volume50
local.description.resumoBackground: BRAF p.V600E is reported in up to 80% of ameloblastomas. Despite the high frequency, the presence of this mutation in different histopathological areas of the tumour has not been investigated. This information has an important role in the use of BRAF p.V600E assessment as an auxiliary tool in the differential diagnosis between unicystic ameloblastoma and other odontogenic cystic lesions, especially when only incisional biopsies are available. Therefore, the purpose of the present study was to investigate BRAF p.V600E heterogeneity in unicystic ameloblastoma. Methods: Five cases of ameloblastoma and two dentigerous cysts were analysed. The regions exhibiting different microscopic characteristics were selected from each ameloblastoma case and manually dissected. TaqMan allele-specific qPCR or Sanger sequencing was performed to determine BRAF p.V600E status. Results: We screened the mutation in a small cohort of UA and no molecular heterogeneity was found. Four cases of ameloblastoma (80%) exhibited BRAF p.V600E in all different areas evaluated. One case did not harbour the mutation in any microscopic region analysed. The BRAF mutation was absent in the dentigerous cysts. Conclusion: Ameloblastomas appear to exhibit a homogeneous profile regarding the BRAF p.V600E no matter what histological feature is observed under light microscopy, suggesting that this molecular test may contribute to establish the correct diagnosis in cases microscopically resembling other odontogenic lesions.
local.identifier.orcidhttps://orcid.org/0000-0002-0199-1884
local.identifier.orcidhttps://orcid.org/0000-0001-8770-8009
local.identifier.orcidhttps://orcid.org/0000-0002-6657-4547
local.identifier.orcidhttps://orcid.org/0000-0001-5150-9227
local.identifier.orcidhttps://orcid.org/0000-0003-2483-8695
local.identifier.orcidhttps://orcid.org/0000-0001-6182-9232
local.identifier.orcidhttps://orcid.org/0000-0003-0046-0026
local.identifier.orcidhttps://orcid.org/0000-0003-2745-2878
local.publisher.countryBrasil
local.publisher.departmentFAO - FACULDADE DE ODONTOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/jop.13155

Arquivos

Licença do pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
License.txt
Tamanho:
1.99 KB
Formato:
Plain Text
Descrição:
Baixar

Coleções

Artigo de Periódico

Logo UFMG