Efeito das proteinas do soro de leite sobre a colonização de Escherichia coli 0157:H7 na mucosa intestinal de camundongos Balb/C.

Abstract

The effect of dietary whey protein concentrate on adhesion and colonization of enterohemorrhagic Escherichia coli O157:H7 in small intestine of Balb/C mice was available. Eight groups of the six females each one, had been separate and had randomly and received diet standard (AIN93G) (control group) and modified (AIN93 modified with addition from the fractions: alpha-lactalbumin and beta-lactoglobulin and total whey protein concentrate - WPC) and water ad libitum per seven days. The blunt groups had received aliquot from 0,5ml of E. coli O157: H7 (ATCC 43895), in the concentration of 7 x 1010 UFC/ml by means of gavage cannula. The animals had been examined clinically and sacrificed, in 8º experimental day, as recommendations of the bioethics committee (CETEA/UFMG). The samples of the intestinal portions had been submitted to the histopathology and morphometry. The statistical analyses had been carried through by the Test t-Student and by test ANOVA ONE WAY including analysis of variance and test of multiple comparison according to Tukey (Software GraphPad Prism 3.0.3® - San Diego HERE). The fractions beta-lactoglobulin and alpha-lactalbumin had protective effect on the intestinal vilosity of the distal part on the jejune and the ileum (p< 0,05), respectively, in Balb/C mice infected by E. coli 0157: H7. On the other hand, the WPC did not demonstrate protective effect on the intestinal vilosity. The whey protein presents great potentialfor the control of intestinal infections caused by E. coli 0157: H7