The Ecto-5′nucleotidase/CD73 Mediates Leishmania amazonensis Survival in Macrophages

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dc.creatorBijay Bajracharya
dc.creatorDeena Shrestha
dc.creatorAndre Talvani Pedrosa da Silva
dc.creatorRicardo Gonçalves
dc.creatorLuis Carlos Crocco Afonso
dc.date.accessioned2023-11-16T21:22:44Z
dc.date.accessioned2025-09-08T23:38:50Z
dc.date.available2023-11-16T21:22:44Z
dc.date.issued2022-02-11
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1155/2022/9928362
dc.identifier.issn2314-6141
dc.identifier.urihttps://hdl.handle.net/1843/61018
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofBioMed Research International
dc.rightsAcesso Aberto
dc.subjectMacrófagos
dc.subjectInflamação
dc.subjectLeishmania
dc.subject.otherLeishmania
dc.subject.otherInflammation
dc.subject.otherMacrophages
dc.subject.otherEctonucleotidases
dc.titleThe Ecto-5′nucleotidase/CD73 Mediates Leishmania amazonensis Survival in Macrophages
dc.typeArtigo de periódico
local.citation.epage10
local.citation.spage9928362
local.citation.volume2022
local.description.resumoEndogenous nucleotides produced by various group of cells under inflammatory conditions act as potential danger signals in vivo. Extracellularly released nucleotides such as ATP are rapidly hydrolyzed to adenosine by the coordinated ectonucleotidase activities of CD39 and CD73. Leishmania is an obligate intracellular parasite of macrophages and capable of modulating host immune response in order to survive and multiply within host cells. In this study, the activity of CD73 induced by Leishmania amazonensis in infected macrophages has been investigated and correlated with parasite survival and infection in vitro. For this, the expression of CD39 and CD73, by flow cytometry, in murine peritoneal macrophages infected with metacyclic promastigotes of L. amazonensis has been analyzed. Our results showed that L. amazonensis-infected macrophages, unlike LPS-treated macrophages, increased CD73 expression. It was also noted that when CD73 enzymatic activity was blocked by α, β-methyleneadenosine 5-diphosphate sodium salt (APCP), macrophage parasitism was significantly decreased. Interestingly, these effects were not associated with the production of TNF-α, IL-10, or nitric oxide (NO). Together, these data demonstrate that L. amazonensis induces a regulatory phenotype in macrophages, which by activating the CD39/CD73 pathway allows parasite survival through the action of immunomodulatory adenosine receptors.
local.identifier.orcidhttps://orcid.org/0000-0003-2476-821X
local.identifier.orcidhttps://orcid.org/0000-0003-1281-5330
local.identifier.orcidhttps://orcid.org/0000-0002-6685-6229
local.identifier.orcidhttps://orcid.org/0000-0002-1127-4483
local.identifier.orcidhttps://orcid.org/0000-0003-2498-4086
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE PATOLOGIA
local.publisher.departmentICB - INSTITUTO DE CIÊNCIAS BIOLOGICAS
local.publisher.initialsUFMG
local.url.externahttps://www.hindawi.com/journals/bmri/2022/9928362/

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