Determination of amino acids in plasma samples of preeclampsia patients by liquid chromatography coupled to high-resolution mass spectrometry

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dc.creatorEliane do Nascimento
dc.creatorHelvécio Costa Menezes
dc.creatorRodrigo Ribeiro Resende
dc.creatorVânia Aparecida Mendes Goulart
dc.creatorZenilda de Lourdes Cardeal
dc.date.accessioned2022-12-30T14:35:47Z
dc.date.accessioned2025-09-08T23:37:30Z
dc.date.available2022-12-30T14:35:47Z
dc.date.issued2019
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.description.sponsorshipOutra Agência
dc.identifier.doihttps://doi.org/10.21577/0103-5053.20190093
dc.identifier.issn1678-4790
dc.identifier.urihttps://hdl.handle.net/1843/48526
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of the Brazilian Chemical Society
dc.rightsAcesso Aberto
dc.subjectQuímica bioinorganica
dc.subjectBioquímica
dc.subjectQuímica inorgânica
dc.subjectAminoácidos
dc.subjectMarcadores biológicos
dc.subjectPre-eclampsia
dc.subjectCromatografia liquida
dc.subjectEspectrometria de massa
dc.subject.otherL-amino acids
dc.subject.otherBiomarkers
dc.subject.otherPreeclampsia
dc.subject.otherLC-HRMS-ESI-IT-TOF
dc.titleDetermination of amino acids in plasma samples of preeclampsia patients by liquid chromatography coupled to high-resolution mass spectrometry
dc.typeArtigo de periódico
local.citation.epage2143
local.citation.issue10
local.citation.spage2136
local.citation.volume30
local.description.resumoThis work presents the application of a simple and sensitive method developed for the simultaneous analysis of the levels of twenty two underivatizated free L-amino acids in plasma samples of preeclampsia patients by high performance liquid chromatography coupled to high-resolution mass spectrometer (LC-HRMS) using hydrophilic interaction liquid chromatography (HILIC) silica column. The linearity was evaluated in the range of 0.009 to 8.018 μmol L-¹ showing coefficients of determination (R²) in the range of 0.999 to 0.911 (p < 0.05). The intra- and inter-day precisions were above of 9.84 and 15.31, respectively. The limits of detection (LOD) and quantification (LOQ) were in the range of 0.003 to 0.683 μmol L-¹ and 0.009 to 2.276 μmol L-¹, respectively. The results of the investigation of free L-amino acids showed that the levels of GABA, Arg, Ser, Leu, Ile, Met, Val, Ala, Gly, Bet, His and Lys in plasma of patients with preeclampsia were significantly different from healthy patients.
local.identifier.orcidhttps://orcid.org/0000-0002-5759-612X
local.identifier.orcidhttps://orcid.org/0000-0002-2003-0739
local.identifier.orcidhttps://orcid.org/0000-0002-3234-8357
local.identifier.orcidhttps://orcid.org/0000-0003-1383-6299
local.publisher.countryBrasil
local.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
local.publisher.departmentICX - DEPARTAMENTO DE QUÍMICA
local.publisher.initialsUFMG
local.url.externahttp://static.sites.sbq.org.br/jbcs.sbq.org.br/pdf/2019-0048AR_BrazWoman.pdf

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