Tetrazolium test to assess the viability of kale seeds

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dc.creatorThais Silva Sales
dc.creatorValter Carvalho de Andrade Júnior
dc.creatorAlcinei Mistico Azevedo
dc.creatorMarcelo Augusto Vieira Santos
dc.creatorSoryana Gonçalves Ferreira de Melo
dc.creatorMarcela Carlota Nery
dc.date.accessioned2023-10-18T11:58:01Z
dc.date.accessioned2025-09-09T00:26:33Z
dc.date.available2023-10-18T11:58:01Z
dc.date.issued2022
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.identifier.doihttps://doi.org/10.1590/2317-1545v44261928
dc.identifier.issn2317-1545
dc.identifier.urihttps://hdl.handle.net/1843/59591
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Seed Science
dc.rightsAcesso Aberto
dc.subjectCouve
dc.subjectCouve -- Fisiologia
dc.subjectCouve -- Sementes -- Viabilidade
dc.titleTetrazolium test to assess the viability of kale seeds
dc.typeArtigo de periódico
local.citation.epage9
local.citation.spage1
local.citation.volume44
local.description.resumoKale (Brassica oleracea var. acephala) has great importance due to its easy propagation, acceptability and nutraceutical properties. The aim of the present work was to make methodological adjustments to conduct the tetrazolium test in kale seeds. Pre-tests were initially carried out to assess the priming time at 20 °C for 10 and 14 hours (times defined by the imbibition curve) and methods of removing the coat of kale seeds (total removal of the seed coat; cut in the distal region to the embryonic axis; longitudinal cut along the longest axis and whole seeds). Subsequently, the most efficient methodologies were evaluated using different concentrations of the tetrazolium salt solution (0.075%; 0.2%; 0.5% and 1.0%) and times (2, 4 and 6 h) of seed immersion in the solution, using four lots. The seeds were analyzed individually and classified as viable or non-viable. The tetrazolium test is efficient for evaluating the viability of kale seeds, providing results correlated with germination. Kale seeds should be primed between paper for 10 hours at 20 °C, and the seed coat should be removed for immersion in a 0.5% tetrazolium salt solution for 4 hours at 30 °C.
local.identifier.orcidhttps://orcid.org/0000-0002-6558-6896
local.identifier.orcidhttps://orcid.org/0000-0002-5010-7725
local.identifier.orcidhttps://orcid.org/0000-0001-5196-0851
local.identifier.orcidhttps://orcid.org/0000-0002-2677-0893
local.identifier.orcidhttps://orcid.org/0000-0001-9464-5827
local.identifier.orcidhttps://orcid.org/0000-0002-9301-2785
local.publisher.countryBrasil
local.publisher.departmentICA - INSTITUTO DE CIÊNCIAS AGRÁRIAS
local.publisher.initialsUFMG
local.url.externahttps://www.scielo.br/j/jss/a/vhHgrRjYMHqpPK7BxMxbm8k/?format=pdf&lang=en

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