Bovine milk extracellular vesicles are osteoprotective by increasing osteocyte numbers and targeting RANKL/OPG system in experimental models of bone loss

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dc.creatorMarina Chaves de Oliveira
dc.creatorBartijn Pieters
dc.creatorPolianna B. Guimarães
dc.creatorLetícia F. Duffles
dc.creatorJoyce Elisa Heredia
dc.creatorAna Letícia Malheiros Silveira
dc.creatorAmanda C. C. Oliveira
dc.creatorMauro Martins Teixeira
dc.creatorAdaliene Versiani Matos Ferreira
dc.creatorTarcilia A Silva
dc.creatorFons Van De Loo
dc.creatorSoraia Macari
dc.date.accessioned2023-05-25T20:15:57Z
dc.date.accessioned2025-09-08T23:30:31Z
dc.date.available2023-05-25T20:15:57Z
dc.date.issued2020
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.3389/fbioe.2020.00891
dc.identifier.issn2296-4185
dc.identifier.urihttps://hdl.handle.net/1843/53961
dc.languagepor
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofFrontiers in bioengineering and biotechnology
dc.rightsAcesso Aberto
dc.subjectVesículas extracelulares
dc.subjectLeite
dc.subjectOsteoporose
dc.subjectObesidade
dc.subjectOsteócitos
dc.subject.otherextracellular vesicles
dc.subject.othermilk
dc.subject.otherbone loss
dc.subject.otherosteoporosis
dc.subject.otherobesity
dc.subject.otherosteocyte
dc.titleBovine milk extracellular vesicles are osteoprotective by increasing osteocyte numbers and targeting RANKL/OPG system in experimental models of bone loss
dc.typeArtigo de periódico
local.citation.volume8
local.description.resumoStudying effects of milk components on bone may have a clinical impact as milk is highly associated with bone maintenance, and clinical studies provided controversial associations with dairy consumption. We aimed to evaluate the impact of milk extracellular vesicles (mEVs) on the dynamics of bone loss in mice. MEVs are nanoparticles containing proteins, mRNA and microRNA, and were supplemented into the drinking water of mice, either receiving diet-induced obesity or ovariectomy (OVX). Mice receiving mEVs were protected from the bone loss caused by diet-induced obesity. In a more severe model of bone loss, OVX, higher osteoclast numbers in the femur were found, which were lowered by mEV treatment. Additionally, the osteoclastogenic potential of bone marrow-derived precursor cells was lowered in mEV-treated mice. The reduced stiffness in the femur of OVX mice was consequently reversed by mEV treatment, accompanied by improvement in the bone microarchitecture. In general, the RANKL/OPG ratio increased systemically and locally in both models and was rescued by mEV treatment. The number of osteocytes, as primary regulators of the RANKL/OPG system, raised in the femur of the OVX mEVs-treated group compared to OVX nontreated mice. Also, the osteocyte cell line treated with mEVs demonstrated a lowered RANKL/OPG ratio. Thus, mEVs showed systemic and local osteoprotective properties in two mouse models of bone loss reflected in reduced osteoclast presence. Data reveal mEV potential in bone modulation, acting via osteocyte enhancement and RANKL/OPG regulation. We suggest that mEVs could be a therapeutic candidate for the treatment of bone loss.
local.identifier.orcidhttps://orcid.org/0000-0002-7423-9802
local.identifier.orcidhttps://orcid.org/0000-0001-7171-3145
local.identifier.orcidhttps://orcid.org/0000-0002-2839-8757
local.identifier.orcidhttps://orcid.org/0000-0003-1052-0854
local.identifier.orcidhttps://orcid.org/0000-0002-6944-3008
local.identifier.orcidhttps://orcid.org/0000-0003-2256-8652
local.identifier.orcidhttps://orcid.org/0000-0002-5851-099X
local.publisher.countryBrasil
local.publisher.departmentENF - DEPARTAMENTO DE NUTRIÇÃO
local.publisher.departmentFAO - DEPARTAMENTO DE ODONTOLOGIA RESTAURADORA
local.publisher.departmentICB - DEPARTAMENTO DE BIOQUÍMICA E IMUNOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE FARMACOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://www.frontiersin.org/articles/10.3389/fbioe.2020.00891/full

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