Investigação dos efeitos cardioprotetores e ansiolíticos do aceturato de diminazeno em modelo de estresse crônico

Abstract

Among the several factors that predispose to cardiovascular diseases, emotional stress stands out. Diminazene aceturate (DIZE) is an antiprotozoan used in veterinary medicine that by acting secondarily as an ECA2 activator increasing the levels of circulating Ang- (1-7), has aroused the interest of researchers for its beneficial effects on anxiety models, myocardial infarction, emotional stress and hypertension. The aim of this study was to investigate the cardioprotective and anxiolytic effects of oral treatment with DIZE in Wistar rats, subjected to chronic stress. The 5 experimental protocols aimed to evaluate the cardiovascular effects of the chronic stress and to evaluate the effects of treatment with DIZE associated with chronic stress on the levels of anxiety, glycemia and integrity of the cardiac muscle, reactivity to stress, cardiac function, morphology of the heart, adrenals and body weight, expression of SERCA2 and phospholamban proteins in the left ventricle and mechanisms of action of DIZE through the evaluation of the activity of the angiotensin-converting enzyme 2 (ECA2) and expression of the Mas receptor. The vehicle-treated group subjected to chronic stress showed lower body weight gain, higher levels of anxiety, lower coronary perfusion pressure and higher heart rate in the isolated heart model, greater heart weight and cardiomyocyte diameter. The group treated with DIZE and subjected to chronic stress showed lower levels of anxiety, attenuation of cardiac reactivity to stress, increased cardiac efficiency and greater activity of ACE2. Together, our data show the deleterious effects caused by chronic stress on the cardiovascular system and suggest that DIZE can contribute significantly to prevent these changes.