Effect of porous tantalum on the biological response of human peripheral mononuclear cells exposed to Porphyromonas gingivalis

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dc.creatorEduardo Frois Temponi
dc.creatorPaulo Eduardo Alencar Souza
dc.creatorGiovanna Ribeiro Souto
dc.creatorLuísa Mourão Dias Magalhães
dc.creatorWalderez Ornelas Dutra
dc.creatorKenneth John Gollob
dc.creatorTarcília Aparecida da Silva
dc.creatorRodrigo Villamarim Soares
dc.date.accessioned2025-04-24T22:07:00Z
dc.date.accessioned2025-09-09T00:36:42Z
dc.date.available2025-04-24T22:07:00Z
dc.date.issued2019-11
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1111/jicd.12472
dc.identifier.issn2041-1626
dc.identifier.urihttps://hdl.handle.net/1843/81836
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of investigative and clinical dentistry
dc.rightsAcesso Restrito
dc.subjectCytokines
dc.subjectPorphyromonas gingivalis
dc.subjectLeukocytes, mononuclear
dc.subjectBehavior
dc.subjectBiology
dc.subjectCell adhesion
dc.subjectTransforming growth factor beta
dc.subjectRANK ligand
dc.subjectTantalum
dc.subject.otherPorphyromonas gingivalis
dc.subject.otherCytokines
dc.subject.otherHuman peripheral mononuclear cells
dc.subject.otherImplantology
dc.subject.otherPorous tantalum
dc.titleEffect of porous tantalum on the biological response of human peripheral mononuclear cells exposed to Porphyromonas gingivalis
dc.typeArtigo de periódico
local.citation.epagee12477
local.citation.issue4
local.citation.spagee12472
local.citation.volume10
local.description.resumoAim: To evaluate biological behavior of human peripheral mononuclear cells (PBMC) in contact with porous tantalum (PT) and Porphyromonas gingivalis (Pg). Methods: Pg was incubated for 8 hours. The groups formed were: PBMC (control), PBMC + PT, PBMC + Pg and PBMC + PT + Pg. Cell viability was evaluated using MTT assay. The morphology and adhesion of PBMC to PT was evaluated using scanning electron microscopy. Expression of interleukin (IL)-10, transforming growth factor (TGF)-β, matrix metallopeptidase (MMP)-9 and receptor activator of nuclear factor-κΒ ligand (RANKL) was determined by enzyme-linked immunosorbent assay. Results: MTT assay revealed that PT did not interfere in the mitochondrial activity of PBMC (P > .05). Scanning electron microscopy showed the adherence of PBMC to PT. IL-10 levels in PBMC + PT were similar to PBMC and lower than PBMC + Pg. TGF-β levels in PBMC + PT were higher than PBMC and PBMC + Pg. MMP-9 levels in PBMC + PT were similar to PBMC and lower than PBMC + Pg and PBMC + PT + Pg. RANKL levels in PBMC + PT were lower than in PBMC. Conclusion: PT did not affect PBMC viability, allowed cell adhesion, reduced expression of RANKL and enhanced TGF-β in comparison with the control group.
local.identifier.orcidhttps://orcid.org/0000-0003-0612-7275
local.identifier.orcidhttps://orcid.org/0000-0001-5166-1982
local.identifier.orcidhttps://orcid.org/0000-0003-3617-8794
local.identifier.orcidhttps://orcid.org/0000-0001-9957-3295
local.identifier.orcidhttps://orcid.org/0000-0002-7586-9996
local.identifier.orcidhttps://orcid.org/0000-0003-4184-3867
local.identifier.orcidhttps://orcid.org/0000-0001-9623-7835
local.identifier.orcidhttps://orcid.org/0000-0001-7698-7532
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/jicd.12472

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