Efficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes

Página do item simplificado
dc.creatorMichele Munk Pereira
dc.creatorAdo Jorio de Vasconcelos
dc.creatorHumberto de Mello Brandão
dc.creatorLuiz Orlando Ladeira
dc.creatorBruno Campos de Carvalho
dc.creatorLuiz Sérgio de Almeida Camargo
dc.creatorNádia Rezende Barbosa Raposo
dc.creatorRaquel Varella Serapião
dc.creatorCarolina Capobiango Romano Quintão
dc.creatorSaulo Rodrigues Silva
dc.creatorJaqueline dos Santos Soares
dc.date.accessioned2022-05-24T12:18:01Z
dc.date.accessioned2025-09-08T22:59:23Z
dc.date.available2022-05-24T12:18:01Z
dc.date.issued2016-09-19
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doi10.1038/srep33588
dc.identifier.issn2045-2322
dc.identifier.urihttps://hdl.handle.net/1843/41917
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofScientific Reports
dc.rightsAcesso Aberto
dc.subjectNanotubos
dc.subjectDNA
dc.subject.otherNanotubes
dc.subject.otherPelucid zone
dc.subject.otherTransfection
dc.subject.otherDNA
dc.titleEfficient delivery of DNA into bovine preimplantation embryos by multiwall carbon nanotubes
dc.typeArtigo de periódico
local.citation.epage10
local.citation.spage1
local.citation.volume6
local.description.resumoThe pellucid zone (PZ) is a protective embryonic cells barrier against chemical, physical or biological substances. This put, usual transfection methods are not efficient for mammal oocytes and embryos as they are exclusively for somatic cells. Carbon nanotubes have emerged as a new method for gene delivery, and they can be an alternative for embryos transfection, however its ability to cross the PZ and mediated gene transfer is unknown. Our data confirm that multiwall carbon nanotubes (MWNTs) can cross the PZ and delivery of pDNA into in vitro-fertilized bovine embryos. The degeneration rate and the expression of genes associated to cell viability were not affected in embryos exposed to MWNTs. Those embryos, however, had lower cell number and higher apoptotic cell index, but this did not impair the embryonic development. This study shows the potential utility of the MWNT for the development of new method for delivery of DNA into bovine embryos.
local.identifier.orcidhttps://orcid.org/0000-0001-8927-6190
local.identifier.orcidhttps://orcid.org/0000-0002-5978-2735
local.identifier.orcidhttps://orcid.org/0000-0002-7405-3928
local.identifier.orcidhttps://orcid.org/0000-0002-4935-6070
local.identifier.orcidhttps://orcid.org/0000-0001-9974-711X
local.identifier.orcidhttps://orcid.org/0000-0001-5271-1048
local.identifier.orcidhttps://orcid.org/0000-0002-4198-5753
local.identifier.orcidhttps://orcid.org/0000-0002-6232-2174
local.publisher.countryBrasil
local.publisher.departmentICX - DEPARTAMENTO DE FÍSICA
local.publisher.initialsUFMG
local.url.externahttps://www.nature.com/articles/srep33588

Arquivos

Pacote original

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
Efficient delivery of DNA into.pdf
Tamanho:
1.98 MB
Formato:
Adobe Portable Document Format
Baixar

Licença do pacote

Agora exibindo 1 - 1 de 1
Imagem de Miniatura
Nome:
License.txt
Tamanho:
1.99 KB
Formato:
Plain Text
Descrição:
Baixar

Coleções

Artigo de Periódico

Logo UFMG