Development of an impedimetric immunosensor for specific detection of snake venom

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dc.creatorRicardo Adriano Dorledo de Faria
dc.creatorVanessa de Freitas Cunha Lins
dc.creatorGiancarlo Ubaldo Nappi
dc.creatorTulio Matencio
dc.creatorLuiz Guilherme Dias Heneine
dc.date.accessioned2023-08-11T14:25:15Z
dc.date.accessioned2025-09-09T00:49:52Z
dc.date.available2023-08-11T14:25:15Z
dc.date.issued2018
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.identifier.doihttps://doi.org/10.1007/s12668-018-0559-7
dc.identifier.issn2191-1649
dc.identifier.urihttps://hdl.handle.net/1843/57733
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofBioNanoScience
dc.rightsAcesso Restrito
dc.subjectEletroquímica
dc.subjectBiossensores
dc.subjectCobra venenosa — Veneno
dc.subjectEspectroscopia de impedância
dc.subjectReações antígeno-anticorpo
dc.subjectImunocomplexos
dc.subject.otherImmunosensor
dc.subject.otherCrofer 22 APU steel
dc.subject.otherSnake venom
dc.subject.otherElectrochemical impedance spectroscopy
dc.titleDevelopment of an impedimetric immunosensor for specific detection of snake venom
dc.typeArtigo de periódico
local.citation.epage996
local.citation.issue4
local.citation.spage988
local.citation.volume8
local.description.resumoSerotherapy is the only approved method to treat victims of snakebites; therefore, it is very important to identify the type of venom implicated in the accident to ensure the most specific antivenom is administered to the patient. In Brazil, the majority of snakebite accidents are from snakes of Bothrops genus. In this work, we developed an immunosensor capable to recognize specifically venoms from Bothrops snakes based on the electrochemical impedance spectroscopy technique. For this, Crofer 22 APU steel was used as transducer substrate functionalized with antibothropic antibodies. The immunosensor was incubated with different concentrations of venoms from Bothrops, Crotalus, and Micrurus in order to evaluate its specificity. The formation of the antigen-antibody immunocomplex at the surface of the transducer substrate increased the leakage resistance in a concentration-dependent manner when the device was exposed to the bothropic venom, while no considerable variation of this parameter could be observed for the venoms of the heterologous genera. In addition to the observed specificity, the sensor proved reusable, since the immersion in an elution buffer permitted many regeneration and charge cycles without considerable loss of activity. The results strongly indicated that the developed immunosensor stands as a promising device to aid in snakebites diagnosis.
local.identifier.orcidhttps://orcid.org/0000-0002-7025-7042
local.identifier.orcidhttps://orcid.org/0000-0002-6357-9553
local.identifier.orcidhttps://orcid.org/0000-0001-9150-3938
local.identifier.orcidhttps://orcid.org/0000-0002-5660-8125
local.identifier.orcidhttps://orcid.org/0000-0003-0707-2691
local.publisher.countryBrasil
local.publisher.departmentENG - DEPARTAMENTO DE ENGENHARIA QUÍMICA
local.publisher.departmentICX - DEPARTAMENTO DE QUÍMICA
local.publisher.initialsUFMG
local.url.externahttps://link.springer.com/article/10.1007/s12668-018-0559-7

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