Perfil metabólico de vacas da raça Holandesa durante o período de transição, locais de colheita e métodos de análise de beta hidroxibutirato.

Abstract

Twelve dairy cows were collected between three weeks to parturition to three weeks after. They was in a free-stall in Inhaúma city, Minas Gerais estate between October and December of 2012. The metabolic profile was compounded with serum non-esterified fatty acids (AGNE), beta-hydroxybutyrate (BHBA), cholesterol (COL), total protein (TP), albumin (ALB), calcium, phosphorus, magnesium as well as the activity serum enzymes aspartate transaminase (AST) and lactate dehydrogenase (LDH). In a group of six animals, four sites were evaluated for blood collection - jugular, mammary and coccygeal veins and tip of tail - to BHBA measurement and comparison of the method of analysis of the electrochemical handset with the laboratory method by spectrophotometry by chemical moist. There were differences in serum NEFA and BHBA between prepartum and postpartum (p <0.05). There was a decrease in the concentration of COL with the approaching birth with subsequent increase (p <0.05). Serum concentrations of minerals, PT and ALB did not differ (p> 0.05). The enzymatic activity of AST and LDH were higher in the postpartum period (p <0.05). Serum BHBA measure in the samples collected from tip of tail, jugular and coccygeal did not differ (p> 0.05) being higher than in the mammary vein (p <0.05). There was no difference between the methods of analysis (p> 0.05). The metabolic profile is an important monitoring tool. The handset is efficient in determining BHBA and can be used blood samples from the jugular and coccygeal veins and from the tip of tail.