Bringing benign ectomesenchymal odontogenic tumours to the lab: an in vitro study using an organotypic culture model

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dc.creatorVictor Coutinho Bastos
dc.creatorNubia Braga Pereira
dc.creatorMarina Gonçalves Diniz
dc.creatorLuciana de Oliveira Andrade
dc.creatorWagner Henriques de Castro
dc.creatorGregory Thomas Kitten
dc.creatorRicardo Santiago Gomez
dc.creatorCarolina Cavalieri Gomes
dc.date.accessioned2025-07-23T20:38:23Z
dc.date.accessioned2025-09-08T22:59:54Z
dc.date.available2025-07-23T20:38:23Z
dc.date.issued2019-02
dc.description.sponsorshipCNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico
dc.description.sponsorshipFAPEMIG - Fundação de Amparo à Pesquisa do Estado de Minas Gerais
dc.description.sponsorshipCAPES - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1111/jop.12812
dc.identifier.issn1600-0714
dc.identifier.urihttps://hdl.handle.net/1843/83789
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Oral Pathology & Medicine
dc.rightsAcesso Restrito
dc.subjectCell culture techniques
dc.subjectCell culture techniques, three dimensional
dc.subjectCementoma
dc.subjectMyxoma
dc.subjectOdontogenic tumors
dc.subjectCell survival
dc.subjectMicroscopy
dc.subject.other3D culture
dc.subject.otherCell culture
dc.subject.otherCemento-ossifying fibroma
dc.subject.otherOdontogenic myxoma
dc.subject.otherOdontogenic tumours
dc.titleBringing benign ectomesenchymal odontogenic tumours to the lab: an in vitro study using an organotypic culture model
dc.typeArtigo de periódico
local.citation.epage179
local.citation.issue2
local.citation.spage174
local.citation.volume48
local.description.resumoBackground: Benign neoplasms exhibit most of the cellular phenomena considered hallmarks of cancer, except the capacity to metastasize. Thus, the elucidation of the mechanisms associated with the progression of benign neoplasms may complement and clarify the mechanisms involved in carcinogenesis. Benign odontogenic tumours often result in facial deformities and morbidities, and have complex pathogenesis, mainly due to the diversity of interactions between the odontogenic epithelium and the ectomesenchyme. Primary cell culture of such tumours is not only difficult to be established and maintained, but also tumour cells lose characteristic cellular morphology. Considering gene expression, growth, migration, proliferation and cellular morphology are controlled by cell-cell interactions and cell-extracellular matrix interactions, cell culture in 3D substrates has gained space as a way to overcome some of the limitations of traditional monolayer cell culture systems. Methods: In this study, fragments obtained from mesenchymal odontogenic tumours were cultured in type I collagen scaffolds. Invasion tests were performed in these models, as well as phenotypic characterization of the cultured tumours. Results: The results obtained for the odontogenic myxoma and the cemento-ossifying fibroma demonstrate a good reproduction of the growth pattern of these tumours under ex vivo conditions. Microscopic evaluation showed maintenance of cell viability in the explants for more than 30 days, without the presence of necrosis. Conclusion: This is the first study involving long-term 3D primary cultures of benign odontogenic tumours, which is expected to support complex approaches to cell and molecular biology, and to serve as an experimental model for testing molecular therapies.
local.identifier.orcidhttps://orcid.org/0000-0003-2745-2878
local.identifier.orcidhttps://orcid.org/0000-0002-6714-3124
local.identifier.orcidhttps://orcid.org/0000-0002-4212-1172
local.identifier.orcidhttps://orcid.org/0000-0002-0536-8790
local.identifier.orcidhttps://orcid.org/0000-0002-4044-9932
local.identifier.orcidhttps://orcid.org/0000-0001-8770-8009
local.identifier.orcidhttps://orcid.org/0000-0003-1580-4995
local.identifier.orcidhttps://orcid.org/0000-0003-0360-4179
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.departmentICB - DEPARTAMENTO DE MORFOLOGIA
local.publisher.departmentICB - DEPARTAMENTO DE PATOLOGIA
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/jop.12812

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