Diagnosis of visceral and cutaneous leishmaniasis using loop-mediated isothermal amplification (LAMP) protocols: a systematic review and meta-analysis

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dc.creatorAstrid Christine Erber
dc.creatorPeter Julian Sandler
dc.creatorDaniel Moreira de Avelar
dc.creatorInes Swoboda
dc.creatorGlaucia Fernandes Cota
dc.creatorJulia Walochnik
dc.date.accessioned2023-12-07T21:39:32Z
dc.date.accessioned2025-09-09T00:57:44Z
dc.date.available2023-12-07T21:39:32Z
dc.date.issued2022-01-24
dc.format.mimetypepdf
dc.identifier.doihttps://doi.org/10.1186/s13071-021-05133-2
dc.identifier.issn1756-3305
dc.identifier.urihttps://hdl.handle.net/1843/61857
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofParasites & Vectors
dc.rightsAcesso Aberto
dc.subjectLeishmaniose
dc.subjectTestes de Diagnóstico Rápido
dc.subject.otherLeishmaniasis
dc.subject.otherCutaneous leishmaniasis
dc.subject.otherVisceral leishmaniasis
dc.subject.otherLoop-mediated isothermal amplification
dc.subject.otherIn vitro diagnostics
dc.subject.otherNeglected tropical disease
dc.subject.otherMeta-analysis
dc.titleDiagnosis of visceral and cutaneous leishmaniasis using loop-mediated isothermal amplification (LAMP) protocols: a systematic review and meta-analysis
dc.typeArtigo de periódico
local.citation.epage16
local.citation.spage1
local.citation.volume15
local.description.resumoSensitive, reliable and fast diagnostic tools that are applicable in low-resource settings, at the point of care (PoC), are seen as crucial in the fight against visceral leishmaniasis (VL) and cutaneous leishmaniasis (CL). Addressing the need for a PoC test, several diagnostic tests, including serological and molecular methods, have been developed and evaluated in the past. One promising molecular method, already implemented for diagnosis of a range of diseases, is the loop-mediated isothermal amplification (LAMP) protocol. In this systematic review and meta-analysis, using a comprehensive search strategy, we focus on studies evaluating the performance of LAMP for the diagnosis of leishmaniasis in humans and other mammals such as dogs, compared with microscopy and/or any other molecular diagnostic method. A meta-analysis, pooling sensitivity and specificity rates and calculating areas under the curve (AUCs) in summary receiver operating characteristic (SROC) plots, was conducted on datasets extracted from studies, grouped by clinical condition and sample type. We found high sensitivity and specificity for LAMP when compared with microscopy and PCR using blood samples, with pooled estimate values of > 90% for all subgroups, corresponding to calculated AUC values > 0.96, except for LAMP compared to microscopy for diagnosis of CL. However, only a limited number of studies were truly comparable. Most of the observed heterogeneity is likely based on true differences between the studies rather than sampling error only. Due to simple readout methods and low laboratory equipment requirements for sample preparation compared to other molecular methods, LAMP is a promising candidate for a molecular (near-)PoC diagnostic method for VL and CL.
local.identifier.orcidhttps://orcid.org/0000-0003-0538-7403
local.publisher.countryBrasil
local.publisher.departmentHCL - HOSPITAL DAS CLINICAS
local.publisher.initialsUFMG
local.url.externahttps://parasitesandvectors.biomedcentral.com/articles/10.1186/s13071-021-05133-2

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