Tissue microarray use for immunohistochemical study of ameloblastoma

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dc.creatorRodrigo Neves-Silva
dc.creatorAlan Roger dos Santos-Silva
dc.creatorFelipe Paiva Fonseca
dc.creatorAdriana Souza de Jesus
dc.creatorHélder Antônio Rebelo Pontes
dc.creatorAndré Caroli Rocha
dc.creatorThais Bianca Brandão
dc.creatorPablo Agustin Vargas
dc.creatorMárcio Ajudarte Lopes
dc.creatorOslei Paes de Almeida
dc.date.accessioned2023-09-14T19:20:41Z
dc.date.accessioned2025-09-09T01:31:16Z
dc.date.available2023-09-14T19:20:41Z
dc.date.issued2016
dc.identifier.doihttps://doi.org/10.1111/jop.12428
dc.identifier.issn09042512
dc.identifier.urihttps://hdl.handle.net/1843/58685
dc.languageeng
dc.publisherUniversidade Federal de Minas Gerais
dc.relation.ispartofJournal of Oral Pathology & Medicine
dc.rightsAcesso Restrito
dc.subjectAmeloblastoma
dc.subjectGenes, Bcl-2
dc.subjectKi-67 Antigen
dc.subjectTissue array analysis
dc.subjectLamins
dc.subject.otherassunto ameloblastoma fao clínica
dc.subject.otherJournal of oral pathology & medicine revista busca dia 28 do 08 agosto ano 2023
dc.titleTissue microarray use for immunohistochemical study of ameloblastoma
dc.typeArtigo de periódico
local.citation.epage711
local.citation.issue9
local.citation.spage704
local.citation.volume45
local.description.resumoBackground: Ameloblastoma is a locally aggressive odontogenic tumor with high rates of recurrence. To better understand the molecular basis of ameloblastoma, tissue microarray (TMA) may represent a useful tool. However, despite TMA has been considered a high-throughput technique for different human neoplasms, it remains to be validated in the ameloblastoma context. Therefore, the objective of this study was to validate TMA for immunohistochemical study of ameloblastoma, determining its most appropriate design. Methods: Forty cases of ameloblastoma were manually distributed in two TMA blocks assembled in triplicate containing 1.0- and 2.0-mm cores (20 cases each). Immunohistochemistry for cytokeratins 14 and 19, and Bcl-2 and Ki-67 was performed, and semiquantitative analysis was performed. Results obtained with TMA sections were compared to their corresponding conventional whole-section slides (CWSS). Results: Kappa statistical test demonstrated that both 1.0- and 2.0-mm cores assessed as duplicate or triplicate significantly correlated with CWSS, with higher levels obtained using Ki67 (k = 0.98, 0.97, 0.88, 0.87) and CK19 (k = 0.62, 0.58, 0.85, 0.85). There was no significant difference between 1.0- and 2.0-mm cores, and between duplicate and triplicate values. 1.0-mm TMA showed a higher index of core loss (33.74% vs. 4.99%). Conclusion: Using a manual arrayer, it was demonstrated that 1.0-mm TMA arranged in duplicate is a valid method for ameloblastoma immunohistochemical study with satisfactory levels of agreement between TMA cylinders and CWSS.
local.publisher.countryBrasil
local.publisher.departmentFAO - DEPARTAMENTO DE CLÍNICA
local.publisher.initialsUFMG
local.url.externahttps://onlinelibrary.wiley.com/doi/10.1111/jop.12428

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